کہنے کو ایک پل بھی تُو مجھ سے جدا نہیں
پر یہ بھی سچ ہے مجھ پہ تُو پورا کھلا نہیں
We are delighted and proud to welcome you to the second issue of Volume 2. Each article received and accepted is an important contribution to the already existing knowledge in the field of Biomedical Sciences. All the editorial team is excited about the progress of PBMJ as an international journal. As editors, we would like to express our heartiest congratulation to the team and welcome to the authors and readers. We are also grateful to the advisory board and managing editors. We hope that PBMJ can promote the academic and applicable research and improve the research activities and collaborations. We are aware of the bumps along the way, but we are determined to keep pursuing the research goals to meet the high quality standards and move forward with great courage. If you have any suggestions to improve, you may write to us as a reader. In the age of technology, we can actively conversate with the readers and get their feedback to improve the quality with their valuable input. PBMJ will continue to serve the Biomedical Sciences as an outlet for high-quality research. This is an exciting time for the journal and we look forward to working with authors, the Editorial board and the team to make PBMJ as a leading source for work in the space.
The current research work was conducted to examine, the isolation and phylogenetic analysis of Avulavirus-1 (AAvV-1) that affected different avian species, in past this virus is known as Newcastle disease virus (NDV) or Avian paramyxovirus-1 (APMV-1) causing Newcastle disease (ND) in both poultry and non-poultry avian species in Azad Jammu and Kashmir (AJK) during 2017-18. AAvV-1s are distributed worldwide and the global risk of ND and there are some needs of safe and consistent approaches to elucidate its epidemiology. Hence, the aim of this study was to pathotyped and genetically characterized AAvV-1 isolated from diseased and clinically healthy poultry and multiple non-poultry avian species in AJK. Genetic and phylogenetic analysis based on complete fusion gene sequences showed that fifteen AAvV-1 isolates obtained from poultry, ducks and peacock belonged to sub-genotype VIIi. Though, one pigeon-origin AAvV-1 isolates were clustered in sub-genotype VI-g and three in recently designated new sub-genotype VI-m of class II. Interestingly, the AAvV-1 isolates of genotype VI and VII had epidemiological connections with viruses isolated recently from different locations in Pakistan. The pigeon-origin isolates had the following two motifs 112-RRKKRF-117 and 112-RRQRRF-117, while all other isolates possess the polybasic sequence of amino acid 112-RRQKR?F-117 at the cleavage site F, which is an important characteristic of virulent strains. Our findings, together with recent AAvV-1 studies, suggest possible virus interspecies transmission between poultry and non-poultry avian species and the potential for an AAvV-1 epidemic in the future. The viruses are prevalent in AJK, this work highlighted the need for continuous and large-scale surveillance studies to control and eradicate the disease.