شرم الشیخ سے قاہرہ واپسی
دس بارہ گھنٹے کی طویل مسافت کے بعد شرم الشیخ سے قاہرہ پہنچے ۔کانفرس کے مندوبین مختلف مقامات پر بس سے اترتے گئے ۔دکتورمجید،دکتورہ رعشہ،دکتورہ شائمہ، دکتوریحی ٰ ایک ساتھ اترے ۔دکتورہ مونا جو الازہر یونیورسٹی میں اردو کی استاد ہیں جنھوں نے ڈاکٹر مبارک علی کی کتاب پاکستانی معاشرے میں گھٹن اور خواتین کے استحصال پر اپنا مقالہ پڑھا تھا ،جس پر سوال و جواب کے سیشن میں میری طرف سے شدید تنقیدی گفتگو ہوئی تھی اور کانفرس کے باقی ایام میں و ہ مجھ سے کنی کتراتی تھیں، نے بھی آج بس سے اترتے ہوئے چہرے پر خفیف سی مسکراہٹ لا کر آواز دی ’’اﷲ حافظ یا دکتورالطاف‘‘ دکتورہ بسنت کا گھر بھی ہماری رہائش سے پہلے آیا ،انھوں نے مجھے دکتورمحمود کے حوالے کیا اور خود اگلے دن شام کو ملنے کا وعدہ کر کے بس سے اتر گئیں ۔دکتورہ ایمان کو لینے ان کے بھائی وقت پر نہ پہنچ سکے تو ہمارے ساتھ ٹیکسی میں سوار ہو گئیں ۔ایمان کا گھر عسکری فلیٹس میں تھا جہاں قدرے خوشحال لوگ رہتے تھے ،میں نے پوچھا کہ آپ کے شوہر فوج میں ہیں ؟اس نے کہا نہیں وہ سعودی عرب میں ملازمت کر رہے ہیں ۔باقی عربوں کی نسبت مصری زیادہ ہنر مند اور جفا کش ہوتے ہیں ۔ان کا المیہ یہ ہے کہ دورِ فراعنہ سے لے کر عصرِحاضر تک مطلق العنانی اور مارشل لائی طرز حکمرانی نے حاکموں کو مالدار اور عام مصریوں کو غریب تر بنا دیا ہے ۔
The development of works in the field of Quranic interpretation has grown rapidly in this age. The work in this field has been written in various major languages of the world such as Arabic, English and Malay. The resulting works contain a lot of information quoted from the works written by previous authors. For example, the work of Tafsir Nur al-Ihsan written by Muhammad Said Omar contains a lot of information quoted from Tafsir al-Jalalyn, Tafsir al-Baydawi and Tafsir al-Jamal. Nevertheless, this study found that the information was widely quoted by the author until there was an inaccurate information being referred to by him. Therefore, a method or system should be practiced so that the information contained in the work of Tafsir Nur al-Ihsan can be managed well as well as avoid the mistakes of readers. One way to manage these resources is to identify the original work referred to by the author. The analysis of Tafsir Nur al-Ihsan used the genetic approach which was published by Goldman in studying the origin of the resources. This study referred the text from Tafsir Nur al-Ihsan and the texts of works that became author's references, which are Tafsir al-Jalalyn, Tafsir al-Jamal, Tafsir al-Baydawi, Tafsir al-Khazin, Tafsir al-Baghawi, Tafsir al-Tabari, Tafsir al-Qurtubi, Tafsir al-Razi, Tafsir al-Nasafi, Tafsir Ibn Kathir and Tafsir al-Tha’labi, and made a comparison of the texts to detect the similarities and differences. The result of the analysis proved that Syeikh Muhammad Sa’id implemented five methods; which are quoting the text in parallel, writing an abstract, removal of some of the words, adding explan-ation, and refuting part of the text. This study also revealed the purpose of Syeikh Muhammad Sa’id while creating his work is to strengthen the translations written. Thus, he successfully strengthens translation when the contents of the text are parallel to the reference. However, in certain places existed a text from the author's references contradicts with the content of Tafsir Nur al-Ihsan, which failed his attempt to strengthen the translation in certain part of the text. Key words: , , .
The main purpose of this research work was to optimize the production of urate oxidase through mutagensis of Bacillus subtilis. The organism was subjected to ultra violet irradiation and chemical mutagenesis. Ethyle methane sulfonate treated B. subtilis (180 minutes) was proved to be the best for optimum production of urate oxidase by 3 log kill/survival curve. Fermentation medium was also optimized, it was found that substrate concentration (0.5%), fermentation period (36 h), pH (8.5), temperature (35 oC), yeast extract (0.3%) and sucrose (2%) enhanced the activity of the parent and mutant derived enzyme. The enzyme was purified by adopting different techniques i.e ammonium sulfate precipitation, ion exchange and gel filtration chromatography. It was observed that mutated enzyme exhibited 97.56 U/mg specific activity with 256.73 fold improvement. The purified urate oxidase was run on SDS-PAGE which determined a single band with molecular weight of 34 kDa. The purified BSM-2 possessed Km and V max value 0.067 M and 133.3 IU mg-1 min-1 respectively. The optimum pH and temperature for catalytic activity were 7.5 and 35oC respectively. The activation energy for formation of ES complex was 43.4 kJ/mol. Enthalpy (ΔH*), entropy of activation and Gibbs free energy demands for urate oxidase inactivation were 30.26 kJ/mol, -106.27 J mol-1 K-1, 62.99 kJ/mol respectively. Barium chloride, potassium cyanide and zinc sulfate decreased the activity of the enzyme. Whereas, sodium chloride (0.6M), potassium chloride (0.4M) and calcium chloride (0.4M) enhanced the enzymatic activity 123%, 117% and 119% respectively.