الحاج مولوی عین الحق اعظمیؒ
افسوس ہے کہ ۹؍ اگست کو الحاج مولوی عین الحق اعظمی کانپور میں رحلت فرماگئے، اناﷲ وانا الیہ راجعون۔ وہ ۱۵؍ فروری ۱۹۱۳ء کو اعظم گڑھ کے ایک گاؤں میں جواب ضلع مؤ میں ہے پیدا ہوئے ابتدائی تعلیم گھر پر ہوئی مولوی کرنے کے بعد انٹرنس پاس کیا اور ۱۹۳۴ء سے اعظم گڑھ میں چمڑے کی تجارت شروع کی جس میں اﷲ نے برکت دی مگر ان کی حوصلہ مند طبیعت اس پر قانع نہیں ہوئی اور ۱۹۵۹ء سے کانپور بھی ان کی جولانیوں کا مرکز ہوگیا، یہاں جاجمؤ میں انھوں نے نیولائٹ ٹینری کی داغ بیل ڈالی اور جب کاروبار میں زیادہ وسعت و ترقی ہوئی تو ۱۹۶۷ء میں یہیں توطن اختیار کرلیا، اب یہ کاروبار اتنا بڑھ گیا ہے کہ ان کے پانچ بیٹے شب و روز اسی میں لگے رہتے ہیں۔
علم و تعلیم سے ان کو بڑا شغف تھا، اکثر دینی مدارس کی مالی امداد کرتے تھے خود مدرستہ الاسلام کے نام سے اپنے گاؤں میں ایک مدرسہ قایم کیا، جس کے سالانہ جلسوں میں مولانا امین احسن اصلاحی اور دوسرے مشاہیر شریک ہوتے تھے، جاجمؤ میں انھوں نے لڑکیوں کی تعلیم کے لیے جامعۃ الزہرا قائم کیا۔
دارالمصنفین کے علاوہ اعظم گڑھ میں علامہ شبلیؒ کی یاد گاریں مدرستہ الاصلاح سرائمیراور شبلی کالج بھی ہیں،تینوں اداروں اور ان کے ذمہ داروں سے حاجی صاحب مرحوم کے روابط تھے، دارالمصنفین میں ان کی آمد و رفت ۱۹۳۵ء سے شروع ہوگئی تھی انھوں نے اس کی فصل بہار دیکھی تھی، علامہ سلیمان ندویؒ اور مولانا عبدالسلام ندویؒ کی مجلس علم و ادب میں بھی وہ باریاب رہ چکے ہیں، کانپور میں مستقل سکونت اختیار کرنے کے بعد بھی جب وہ اعظم گڑھ آتے تو یہیں قیام کرتے مجھ پر ان کا خاص لطف و کرم رہتا، جب...
Background and Aims: The study identified the risks of increased screen time on generation z considering two parameters i.e., insomnia and obesity and the moderating role of self-control.
Methodology: A cross-sectional study was conducted by convenient sampling technique. The survey only included people who were born between 1997 and 2002. The analysis of the effects of variables took into account only a few parameters. Discriminant validity and convergent validity was used to check reliability and validity while path coefficient analyzed the hypothesis.
Results: Binge-watching has positive significant impact on insomnia and obesity (p<0.05), and the self-control strongly effect the relationship of binge-watching, obesity and insomnia as a moderator (p<0.05).
Conclusion: Binge-watching caused insomnia and obesity while self-control in direct relation to obesity and insomnia controlled the prevalence rate. The moderating role of self-control showed that insufficiency of self-control during binge-watching led to obesity and insomnia. The outcome indicated that Generation Z needs to be encouraged to practice self-control through public health initiatives, social media, seminars, workshops, etc. At the same time, people need to be made aware of how binge-watching affects their health.
Keywords: Insomnia, obesity, self-control, screen time, technology, mortality, exercise.
Oxidative stress reflects an imbalance between production of oxidizing species and their removal through antioxidant system. This imbalance is harmful for biological macromolecules like proteins, lipids and DNA. Oxidative stress has been implicated in pathophysiology of many diseases. To counteract the harmful effects produced due to over production of reactive oxygen species, living organisms are equipped with antioxidant defense mechanism. Paraoxonases (PON) are calcium bound enzymes having antioxidant properties. The PON family constitutes three isoforms including PON1, PON2 and PON3. Among these, PON1 is the most studied member with defined antioxidant property. Reduced PON1 activity has been reported in various disorders including Cataract. Cataract is third most prevalent age related disease responsible for blindness throughout the world. One of the key factors involved in progression of cataract formation is oxidative stress. Another possible contributor is accumulation of advanced glycation end products (AGEs) leading to non-enzymatic glycation of macromolecules particularly proteins. AGEs formation is not only accelerated in presence of hyperglycemia but also triggers production of reactive oxygen species leading to glycoxidation. Thus, interplay of both oxidative imbalance and glycation may play critical role in etiology of cataract. Objective of current study was to explore biological properties, clinical implications and role of Paraoxonase as oxidative biomarker. We chose cataract as a model disease to examine PON family members. Partial purification of PON enzyme was performed using human plasma through affinity and gel filtration chromatography. Partially purified samples were further subjected to SDS-PAGE and MS analysis. The fraction with highest PON1 activity was found to be albumin through mass spectrometric analysis. Our studies confirm that PON1 has a greater affinity for albumin and may exist in association with albumin. PON gene polymorphism has been studied to examine association between genetic variant with incidence of diseases. In current study, we examined PON gene polymorphism for PON1 (L55M & Q192R); PON2 (C311S & G148A) and PON3 (C133A) in normal and cataract subjects using tetra primer ARMS-PCR and RFLP followed by agarose gel electrophoresis. Polymorphic sites 192R of PON1 and G148 of PON2 were found to be more frequent in cataract patients as compared to normal xi individuals suggesting these alleles to be disease specific in studied samples of Pakistani origin. Combine genotype analysis revealed L55/Q192 of PON1 and C311S/ 148A of PON2 in healthy individuals while G148/311S of PON2 among cataract subjects to be significant. PON1 L55M, PON2 C311S and PON3 did not show significant association with cataract. To explore the impact of glycation on PON1 and its biological activity, we incubated plasma from normal and cataractous subjects with sugars (glucose, fructose & galactose) for 15 days. Enzymatic activities (Paraoxonase and arylesterase) along with oxidative stress markers MDA and total ROS were measured. Time dependent structural changes in response to glycation were monitored through spectroscopic analysis while level of glycation was measured by AGEs quantification through ELISA. Our results indicated significant decrease in paraoxonase and arylesterase activities together with substantial increase in MDA and total ROS levels among all incubated samples. Most potent sugar was found to be fructose followed by galactose than glucose. The degree of glycation was found to be in order of fructose>galactose>glucose. To confirm the effect of fructose on PON1 activity, pure PON1 was incubated with fructose which showed 95% decline in PON activity as compared with respective control. In conclusion, our study highlights impact of polymorphism and genetic variation among PON family members in studied groups, biological role of PON1 as an antioxidant in response to glycation and implication of glycation on PON1 activity.