صبحِ قفس کا جمالیاتی پہلو
سر زمین پاک پتن کی عظمت ورفعت کے سے کیسے انکار ہو سکتا ہے۔ یہی وہ مخزن ومعدن تصوف ہے جس میں پائے جانے والے لعل وگہر کسی کو مفلوک الحال یا تشنہ لب نہیں رہنے دیتے۔ فیوض وبرکات سے مالامال اس خطہ تحریم سے محبت کرنے والا کبھی کسی احساس محرومی کاشکار نہیں رہ سکتا۔ خواہ وہ اس سے ہزاروں میل دور ہی کیوں نہ ہو مگر جو سعادت منداپنے صبح و مسا دامن حضرت گنج شکرؒ کے ساتھ وابستگی میں گزاررہا ہو یقینا اس کا طائر تخیل اوج سماء کی جانب ہر وقت محو پرواز رہتا ہے۔ تائب نظامی انھی خوش مقدر لوگوں میں شمار ہوتے ہیں جو ہر لمحہ مزار گنج شکرؒ کی تابش سے اپنے دل ونگاہ کو منور کیے ہوئے ہیں اور پھر خوش قسمتی سے اگر انسان شاعر اور ادیب بھی ہو تو یہاں قیام کا لطف دوآتشہ بلکہ سہ آتشہ ہو جاتا ہے۔ میں دل کی گہرائیوں سے یہ محسوس کرتا ہوں کہ صاحب مزارؒ کے تلطف اور نوازش سے تائب نظامی کے شعری وادبی حوصلوں کو نیاولولہ اور عزائم کو تخلیق کے نئے اُفق عطا ہوتے ہیں۔ صبحِ قفس’’عروض‘‘ کی ایک ایسی دلآویز ہے جو ’’گلہائے رنگارنگ سے ہے زینت چمن‘‘ کا دل پذیر منظر پیش کر رہی ہے۔ میں اس حقیقت کا اظہار کسی تصنع یاریا کے بغیر کر رہا ہوں کہ قدرتِ کاملہ نے اس ’’تلمیذخاص‘‘ کو ردیف قافیہ ، اوزان و بحور اور بندش الفاظ پر جو دسترس عطا کر رکھی ہے اس کی داد میرے امکان میں نہیں۔
غزلیات پر مشتمل صبح قفس کا آغاز حسب روایت حمدو نعت سے ہوتا ہے۔ شاعر ان اصناف کی نزاکتوں سے کماحقہ آگاہ ہیں۔ وہ حمد و نعت کی گہرائی وگیرائی سے اچھی طرح شناسا ہیں۔ حمد کا ایک شعر الوہیت رب...
Natural Sciences learning laboratory activities are an integral part of teaching and learning activities, This shows how important the role of laboratory activities to achieve the goals of science education. Through laboratory activities, students will be given the opportunity to encourage curiosity and the desire to try, test and reason the theories obtained. The purpose of this study was to analyze the management of a science laboratory in SMP Negeri 4 Sojol. The research method uses qualitative methods and descriptive analysis. The research subjects were the head of the laboratory and the science subject teacher and the students at SMP Negeri 4 Sojol. Data collection techniques through observation, interviews and student learning outcomes. Based on the results of the study, the availability of natural science practicum tools in SMP Negeri 4 Sojol, the average availability of practical tools and materials 68.33% classified as complete categories, the average presentation of the feasibility of practical tools and materials 90.67% included in the excellent category based on the Ministry of Education number 24 of 2007. The effectiveness of practicum implementation in SMP Negeri 4 Sojol is classified as effective category with an average presentation of practicum at 85%. Coupled with an average increase in cognitive competencies average preetest 58 increase in posttest by as much as 87 in addition to the average value of competence in the areas of psychomotor average preetest obtained 60 and posttest 90 showed a significant increase competency knowledge and skills of learners. Success in the cognitive domain will also have a positive impact on the development of the psychomotor domain (skills) of students. Management of laboratory facilities and infrastructure planning in improving the quality of learning in schools must be planned by the head of the laboratory, planning science teachers by preparing learning tools and identifying the subject matter to be practiced in the laboratory, laboratory administration governance supported by good organization from the leadership of policy makers in school. Implementation of laboratory facilities and infrastructure management in improving the quality of learning in schools including procurement, inventory, storage, structuring, use, and maintenance. Supervision carried out by the principal is in accordance with the provisions, to be more efficient the school should make a program or schedule of supervision processes to be more controlled and run well and smoothly.
Extended-spectrum b-lactamases (ESBLs) are enzymes that can hydrolyze extended- spectrum cephalosporins and monobactams. ESBL-producing Klebsiella pneumoniae are responsible for serious morbidity and mortality among paediatric patients. This study aimed to determine the frequency of ESBL-producing K. pneumoniae, phenotypic characterization techniques and antimicrobial resistance pattern. The study was also established to determine the molecular characterization of blaSHV, blaTEM, blaCTX-M genes which are responsible for ESBL-mediated antibiotic resistance. The study was conducted at The Children’s Hospital & Institute of Child Health, Lahore, Pakistan during May 2010 to February 2012. The molecular studies of blaSHV, blaTEM, blaCTX-M and integron genes were performed during October 2012 to April 2013 at the Microbiology and Immunology Department, The University of Melbourne, Australia. Various clinical samples were collected and studied from paediatric patients, including blood, central venous pressure line, cerebrospinal fluid, ear swab, endotracheal tube, peritoneal dialysis catheter, pleural fluid, pus, tracheal secretion, urine and wound swab. The organisms were identified using various biochemical tests and the API 20E system. ESBL production was determined using double disk synergy test (DDST) and Clinical and Laboratory Standards Institute (CLSI) confirmatory test. The antimicrobial resistance pattern of ESBL-producing K. pneumoniae was determined using Kirby-Bauer disc diffusion method with various antibiotic groups. The target genes were amplified and DNA sequencing was performed for blaSHV and blaTEM genes to find out the mutations responsible for ESBL genotype. Screening of 710 K. pneumoniae isolates showed 214 (30.1%) were ESBL screen positive K. pneumoniae. The CLSI confirmatory test showed significantly greater sensitivity (p<0.0001) compared to DDST. There were 82 (38.3%) neonates infected with ESBL K. pneumoniae and 152 (71.0%) of the total cases were males. The most common sources of ESBL K. pneumoniae were blood (117; 54.7%) and urine (46; 21.5%). Of the 214 cases, 92 (43.0%) cases were isolated from Neonatal Nursery Unit and (47; 22.0%) Nephrology. Patients presented with various symptoms such as fever (125 cases; 58.4%) and respiratory distress (104 cases; 48.6%). Important interventions given to the patients included intravenous line (209 cases; 97.7%), urinary catheters (46; 21.5%) and endotracheal tube (18; 8.4%). The outcome of the patients showed the successful discharge of 127 (59.0%) patients after treatment while there were 56 (26.0%) cases of mortality and 31 (15.0%) left against medical advice (LAMA). There was no significant correlation (p=0.1396) found between length of stay and mortality of the patient. Neonates infected with K. pneumoniae had a significantly higher chance of mortality than the older age groups (p=0.0140), while there was no association of outcome (p=1.0000) between the two genders. A higher mortality rate (p=0.0005) was seen among the septicemic patients. The mortality rate was significantly higher (p=0.0013) in patients who presented with respiratory distress symptoms. An antibiotic resistance profile of ESBL-producing K. pneumoniae was performed against 18 antibiotics. All ESBL K. pneumoniae isolates were resistant to ceftazidime, ceftriaxone, cefotaxime and cefuroxime. The antibiotics that K. pneumoniae were most resistant to, include co-amoxiclav (212; 99.1%), cefpodoxime (210; 98.1%), co-trimoxazole (207; 96.7%), gentamicin (201; 93.9%), tobramycin (199; 93.0%), aztreonam (192; 89.7%), cefepime (171; 79.9%) and amikacin (164; 76.6%). Only 41 (19.2%) isolates were resistant to cefoxitin and 96 (44.9%) showed medium level resistance to ciprofloxacin. Only one (0.5%) isolate showed resistance to imipenem and meropenem. The number of isolates displaying resistance to sulbactam-cefoperazone and piperacillin-tazobactam were 13 (6.1%) and 7 (3.3%), respectively. The number of antibiotics to which K. pneumoniae were resistant in each patient were compared in patients with (n=67) or without (n=147) history of antibiotic use in the last three months. No significant difference (p=0.5298) found between the two groups. Amplification and analysis of bla genes showed the majority of K. pneumoniae isolates carry the blaSHV (99.5%), blaTEM (93.0%) and blaCTX-M (99.0%) genes. All of the TEM genes isolated in this study were wild type TEM-1 β-lactamases. The ESBL type SHV detected in the present study were SHV-28 (19.2%), SHV-12 (5.2%) and SHV-110 (0.5%), while non-ESBL type SHV were SHV-1 (20.2%), SHV-11 (31.5%), SHV-42 (1.9%) and SHV-27 (1.4%). The CTX-M-1 group β-lactamases was identified in 99% of the strains. K. pneumoniae isolates in the present study were also studied for the presence of an integrase gene and it was found that 94.9% of isolates had a class 1 integrase, while the class 2 and 3 integrase genes were identified in 1.4% and 0.9% of isolates, respectively. This is the first study conducted on clinical isolates of ESBL-producing K. pneumoniae among paediatric patients from a tertiary care paediatric hospital of Pakistan. The high prevalence of ESBL-producing K. pneumoniae among paediatric patients is responsible for prolonged hospital stay and an increased financial burden on parents and the government. Cephalosporins, monobactams, aminoglycosides and sulfonamide drugs do not prove to be a good choice for the treatment of ESBL-producing K. pneumoniae infections to high rates of resistance to these antibiotics. This study recommends the use of carbapenems, sulbactam-cefoperazone and piperacillin-tazobactam for the treatment of ESBL K. pneumoniae infections but they should be used as a last resort following culture and susceptibility testing. It is being recommended that a stricter infection control policy should be implemented to control the horizontal transfer of blaSHV, blaTEM, blaCTX-M genes and integrons in clinical isolates of K. pneumoniae and other bacteria.