مولانا حبیب الرحمن لدھیانوی
یہ سطریں زیر تحریر تھیں کہ مولانا حبیب الرحمن صاحب لدھیانوی کی وفات کی خبر ملی، مرحوم ملک و ملت کے پرانے خدمت گزار، مجلس احرار کے مشہور لیڈر اور جنگ آزادی کے ممتاز مجاہدین میں تھے اور اس راہ میں قید و بند کی مصیبتیں جھیلیں، کانگریس سے ان کا تعلق بہت قدیم تھا، جو ہر زمانہ میں برابر قائم رہا، ہندوستان کی تقسیم اور اپنے وطن لدھیانہ کی تباہی کے بعد دلی میں متوطن ہوگئے تھے، ان کی عمر کا بڑا حصہ قوم و ملک کی خدمت میں گزرا طبعاً بڑے خاکسار متواضع، فیاض اور مہمان نواز تھے، دارالمصنفین کے لوگوں سے بڑا اخلاص رکھتے تھے، جب مشرقی اضلاع کی جانب آنا ہوتا تھا تو دارالمصنفین ضرور آتے تھے، ان کی صحت عرصہ سے خراب تھی، ابھی تھوڑے ہی دن ہوئے آنتوں کا آپریشن ہوا تھا، اس سے تو صحت یاب ہوگئے تھے، مگر ۲؍ ستمبر کو قلب کا دورہ پڑا اور دفعتہ انتقال کرگئے، اﷲ تعالیٰ اس پرانے خادم قوم کو اپنی رحمت و مغفرت سے سرفراز فرمائے۔ (شاہ معین الدین ندوی، ستمبر ۱۹۵۶ء)
Aim To explore the apparent lack of personal concern for the welfare of others in China? To develop concepts to understand this social phenomena in natural settings using ethnographic and participatory research. Research Question How can the knowledge derived from qualitative research be used to improve the welfare of the poor in Shanghai? Ethics The study was carried out within the guidelines of the declaration of Helsinki. The study was characterised by anonymity, beneficence, non maleficence, and the maintainence of the dignity of participants Method Collection of data. The principle data source was “observation” spot observation”(Baksh 1990)”, participant observation”(Hammersley and Atkinson 1983)vignettes(Finch 1987, Sani Bin Gabi 1990), oral history(narrative) Conclusion The study was conducted ethically. It was a worthwhile study attempted to deal with current problems. The publication of this paper can be used to stimulate further enquiry into the problem of those in need of social welfare in Shanghai and China, in the hope it will improve services where they are needed. Suggested solutions In a country where intergenerational family ties are so strong and “guanxi” exists it is paradoxical there is little empathy for others. Confuscian values, changed family beliefs, and education at school and university may assist in increasing empathy. Learnt conditioning, to ignore or dissociate, from another human being in need of help, can be slowly overcome by implementing universal and improved changes in societal living conditions. This can be achieved by improving social welfare programs, spearheaded by a compassionate government. There is a view that it is an egregious and transparent fiction, to promulgate and publically promote, the philosophies and policies of communism, yet allow these curable social ills to remain. ”Rex ipsa Loquiter”. The healthy should help the sick, the rich should help the poor and the employed should help the unemployed, Proportionately, in accordance with what they have they should give. The promotion of a “ forward thinking, humane, listening and open society, rather than a closed, narrow, opaque, inward looking “weltanschaung” would assist societal reform. We “bystanders”, we must all try to imagine we are in the place of those in need, and feel aas they do, in their situation. We must follow the advice of Mencius (372-289BC) and develop and use “empathy” As Mencius said, ”a developed human heart is the basis of a moral life”
Environmental contamination due to improper dumping of industrial effluents and its toxicological manifestations has been recognized as major cause of public health risks, especially in developing countries. It has urged the scientific community to focus on various approaches to remedify their impacts.Keeping in view, the costs and generation of toxic byproducts related to conventional remediation strategies, there is a continuous need to look for environmentally safe technologies. Biological techniques (bioremediation) using microbial biomass and their biomolecules/products offer better treatment of contaminants compared to physical and chemical methods. Such potentials of biofilm forming bacteria and their biopolymers were investigated in present work. Bacteria from biofilm samples were isolated and screened for biofilm formation and decolorization of chemically variable dyes. All bacterial isolates were Gram negative, facultative anaerobes and mesophiles. They conferred resistance to multiple heavy metals including cadmium (100-1000 mg L-1), chromium (7001000 mg L-1), copper (600-1000 mg L-1), lead (1000 mg L-1), iron (1000 mg L-1) and nickel (200-600 mg L-1). Selected isolates with potentials to degrade/transform xenobiotic compounds were characterized at molecular level and identified as Staphylococcus sp. MB377, Bacillus subtilis MB378, Klebsiella oxytoca MB381 and Klebsiella pneumoniae MB398.Bacterial isolates showed the capability of decolorizing and degrading different dyes (Eriochrome black T, Congo red, Malachite green) in the presence and absence of glucose. Bacillus subtilis MB378 was most efficient in degrading these dyes up to 65-90% in presence of glucose. Optimum conditions for decolorization of Congo red dye were pH 5 (Klebsiella pneumoniae MB398), Eriochrome black T dye pH 8 (Bacillus subtilis MB378) and Malachite green dye pH 9 (MB392). While highest decolorization rates of Congo red dye were observed at 37˚C (Klebsiella pneumoniae MB398), Eriochrome black T dye at 30˚C (Bacillus subtilis MB378) and Malachite green dye at 30˚C (MB392), respectively. UV-VIS spectrophotometric & FTIR analysis, high performance liquid chromatography (HPLC) and gas chromatography (GCMS) further confirmed the possible degradation/transformation of dye molecules into respective metabolites.Assessment of simultaneous removal of dye and selected metals by biofilm forming bacteria showed efficient decolorization of Malachite green dye in presence of cadmium, copper and xxiv chromium (up to 79.65 & 88.07%, 79.35 & 93.18%, and 81.63 & 90.32%) by Staphylococcus sp. MB377 and Bacillus subtilis MB378, compared to individual dye molecules. Bioaccumulation yield of copper in combination with Malachite green dye was 30.88 mg L-1 (Staphylococcus sp. MB377), 31.22 mg L-1 (Bacillus subtilis MB378), while for chromium it was 29.88 mg L-1 (Bacillus subtilis MB378). Maximum chemotactic indices were recorded in the presence of glucose for Congo red by MB387 (1.37), Methyl orange by MB391 (0.97), Malachite green by MB391 (0.87) and Eriochrome black T by MB377 (0.74). Highest chemotactic potentials of Klebsiella oxytoca MB381 towards chromium (83.14), Staphylococcus sp. MB377 towards lead (81.23), MB392 towards cadmium (0.74) and Staphylococcus sp. MB377 towards copper (0.47) were recorded through capillary assay in the presence of glucose, while chemotactic response towards dyes and metals declined in the absence of glucose. Biopolymeric substances (EPS) were rich in carbohydrate contents and also harbored fairly good antimicrobial activities against target bacteria with inhibition zones ranging between 0.8-1.91 cm. FTIR spectroscopic analysis showed that these polysaccharides were comprised of proteins, carbohydrates, alkyl halides, nucleic acids, various hydrophilic and hydrophobic functional groups. SEM and XRD analysis revealed compact and crystalline nature of EPS. EPS from Staphylococcus sp. MB377, Klebsiella oxytoca MB381 and Klebsiella pneumoniae MB398 formed 67.71, 61.25 and 60.46% stable emulsions. EPS from Klebsiella pneumoniae MB398 gave highest emulsion retention values for Canola oil (1.330±0.045), Kerosene oil (1.261±0.251) and n-Hexane (0.794±0.120). EPS from Bacillus subtilis MB378 removed 96.09% of cadmium in combination with Malachite green, with an adsorption rate of 0.98 mg g-1. While 90.86% of copper removal in the presence of Malachite green dye was observed for EPS from MB392, compared to individual metal ions. EPS from Staphylococcus sp. MB377 removed 85.10 and 84.76% of Malachite green in combination with cadmium and chromium compared to dye molecules alone. FTIR spectra further confirmed that the reactions and complexation of dye in presence of metals with charged or ionizable functional groups of EPS had facilitated their adsorption and accumulation on to the surfaces of EPS. Complete genome sequencing of Staphylococcus sp. MB377 and Bacillus subtilis MB378 showed that each genome possessed significantly diverse structural and functional features. Both these strains had multiple genes involved in biofilm formation, adhesion, xxv exopolysaccharide production and chemotactic responses. Genes for metal resistance (Cd, Cr, Cd/Zn/Co, Cu) and reduction of arsenic were also annotated in the genomes of Staphylococcus sp. MB377 and Bacillus subtilis MB378, respectively along with multidrug efflux and transporting systems. These strains also possessed wide range of metabolic activities for effective catabolism and detoxification of xenobiotic compounds. Stress induced/response proteins were indicative of defense mechanisms and ability of bacteria to survive in diverse environmental conditions. Annotated genes encoding for various enzymes (dioxygenases, acylphosphatases, nitroreductases, tautomerase, laccase-like multicopper oxidase and oxidoreductases) indicated the potentials of these strains for utilizing and degrading different aromatic compounds and dyes.The diversity and potential of biofilm forming bacteria to adapt in diverse environmental conditions was recognized in present study. The results further supported present bacteria and their biomolecules as most potential/effective candidates for the remediation of polluted sites.