عیدی
جب لوگ آسمان کی وسعتوں میں
ایک خم دار دھندلی سی لکیر تلاش کر رہے تھے
جو سب کے لیے باعث ِ مسرت تھی
مگر کسے خبر تھی
وہ شام میرے لیے غموں کے طوفان لانے والی ہے
اس لکیر کو دیکھتے ہوئے
میرے تصور میں بھی ایک نقطہ سا پھیلنے لگا
جو ایک مانوس صورت کا روپ دھار رہا تھا
چند قیمتی اذیت دہ لمحات میں ڈھلتی صورت
With the current international competition among global companies, Business English as a Lingua Franca (BEFL) has become a necessity. As for one, Boardwalk Direct Selling Company recognizes the adoption of the BEFL concept within the organization to equip its workforce with adequate English language skills at par with global standards. This study aims to assess the organization’s current English proficiency and the readiness of its employees to embrace BEFL. This also presents the major English language skills areas that need improvement through training intervention. A stratified sampling method is utilized to extract data via an online survey. Respondents are strategically chosen to represent different strata such as organizational departments or groups, job levels, tenure, and age. A convenient size of 34 respondents participated in this study. Generally, respondents acknowledge the importance of the English language skill set in their job functions and as criteria for their career growth. Half of the sampling population affirms their English language proficiency. However, the study reveals that Boardwalk employees are willing to subject themselves to improving their English skills, most particularly in speaking and writing aspects. Given their willingness, the employees recommend that the company strengthen its BEFL trainings across the organization. Moreover, with the current setup of mostly working from home due to COVID restrictions, majority of the employees prefer online learning.
Leishmaniasis, a worldwide prevalent disease, is still enjoying the ruling with no proper medication; and to add to this current gloomy scenario the disease causing parasite Leishmania is becoming resistant to the ongoing medication that is being practiced now a days. Hence, the need is to search for reasonable, safe and targeted drugs; the present research is one such effort in this direction. To begin with, Leishmanolysin (GP63), zinc metalloprotease, expressed over the surface of Leishmania species was selected as drug target due to its virulence and reason for parasite resistance. A library of benzimidazole derivatives (1-37) was synthesized and screened for its antileishmanial potential against L. major. All the compounds were found potent antileishmanial with IC50 values in the range of 0.62-0.92 μg/mL as compared to amphotericin B (standard drug) IC50 value 0.56 μg/mL. 2-(Thiophen-2-yl)-1H- benzimidazole (19) and 2-(1H-indol-3-yl)-5-nitro-1H-benzimidazole (34) were identified as the lead compounds of the library with IC50 value of 0.62 μg/mL. ADMET properties of the entire library were also predicted by using ADMET PredictorTM and were observed to be safe. Molecular docking studies carried out on all the members of library and amphotericin B by using MOE software, indicated that the most active compounds fitted at the centre of binding pocket of GP63 built by amino acid residue His264, His268, His334 and Zn578. On the basis of molecular docking results, receptor based pharmacophore model was built containing three Aro|Hyd features and one Acc&ML feature. This pharmacophore model was used to design new scaffolds for antileishmanial compounds. Four libraries, 2-(2- aryl/heteroarylbenzimidazol-1-sulfonyl)anthraquinones (38-69), N-(heteroaryl)-anthraquinon-2- sulfonamides (70-95), aryl anthraquinon-2-sulfonates (96-111) and N-(anthraquinon-2-sulfonyl)-amino acid methylesters (112-123) were designed and all the cmpounds were found as hit by pharamocophoric search. Their antleishmanial activities were predicted by QSAR model; built by MOE software by selection of 94 descriptors and partial least square (PLS) method on experimental antileishmanial activity of 37-mebered library and amphotericin B, validated by internal and exernal test sets with correlation coefficient (R2) 0.7762. All the compounds belonging to four libraries (38-69, 70-95, 96-111 and 112- 123) were found potent antileihmanial with predicted activity in the range of 0.5435-0.9940. All the compouds were observed safe according to predicted ADMET properties and Lipinski’s rule of five (Ro5). Later, these four designed libraries were synthesized and characterized by physical constants and spectroscopic techniques for onward screening for their antileishmanial potential against L. major by using amphotericin B as standard control which confirmed that all the compounds were potent antileishmanial. Compliance of the predicted activity by QSAR model with observed activity from in vitro antileishmanial activity resulted in identification of the same lead compounds in each library 38-69, 70-95, 96-111 and 112-123 i.e. 2-(5-Nitro-4-methoxyphenyl-1H-benzimidazol-1-sulfonyl)anthraquinone (61) (predicted activity 0.6794, IC50 0.67 μg/mL), 2-(1H-benzo-1,2,3-triazol-1-sulfonyl)anthraquinone (91) (predicted activity 0.5579, IC50 0.57 μg/mL), 2-(1H-pyrazol-1-sulfonyl)anthraquinone (90) (predicted activity 0.5435, IC50 0.58 μg/mL), benzyl anthraquinon-2-sulfonate (100) (predicted activity 0.7615, IC50 0.76 μg/mL) and N-(anthraquinon-2-sulfonyl)-2-phenylglycine methylester (123) (predicted activity 0.7305, IC50 0.75 μg/mL). Pharmacophore based molecular docking studies carried out on all the eighty six compounds on GP63 by MOE software showed hydrophobic interactions, hydrogen bonding and metal ligation interactions with His268, His264, His334 and Zn578, respectively. This entire set of experiments in both dry and wet labs led to a successful designing of a variety of anthraquinon-2- sulfonamides as a novel scaffold having strong antileishmanial effect.