اُس نے تو کیا چاہے وہ اقرار نہیں تھا
ایسا بھی نہیںمجھ سے اُسے پیار نہیں تھا
اب شکل مری مجھ سے بھی تو ملتی نہیں ہے
ایسا تو کبھی مَیں اے مرے یار نہیں تھا
The action code of Islam is impartial and strong. All human beings, disabled and abled have the right of gaining justice and bound to giving justice. Because of disableness impartial behavior is against the Islamic justice code. The “good behavior” with disabled persons is the basic law of disableness in Islam. The “good behavior” with disabled persons in Islam is not the result of mercy and pity but is the result of that right of taking equal to the life of common people which is due to the nation and community. Hence the rights of disabled persons are the result of those requirements which are created due to disableness. The meaning which we take conventional is “complete worthlessness” but it means in Islam weak and feeble. That is to say disable person is able to work but he has less ability. Or he has no ability to do one job but has the ability of high rank to do other jobs. The Holy Prophet (Peace be upon him) introduced first time this principle of disableness. The Arabs mean the word “disableness” complete uselessness. But the Holy Quran exempted the persons from Jehad not taking part in Tabuk battle because of disableness giving them the name of feebles. Islam does not appeal for mercy with disabled persons but advised to behave well with them and condemns also the injustice of society with them. Islam orders to perform one’s duties to others. Islam not only stresses on the performance of duties but also gives instructions in this connection.
Cardiovascular diseases are one of the leading causes of mortality in Pakistan with prevalence rate of 19 %. Digoxin, a cardiac glycoside with positive inotropic effect is most commonly prescribed to cardiac patients; involving therapeutic monitoring of drug in serum/plasma through lengthy immunoassay procedures in practice. Also, the detection facility is restricted to private labs in major cities of Pakistan. Nanotechnology with the help of nanomaterials as compared to conventional lengthy procedures offers robust, patient centered on chip methodologies for detection of therapeutically important drugs in serum/plasma. The aim of the current study was fabrication of microfluidic device offering on chip QDs based detection of clinical range of digoxin (0.8-2.0 ng per ml) in custom-made optical setup. To achieve the said objectives, sequence of experiments were conducted. It was imperative to delineate the effect of pH of media on the fluorescence of zinc sulphide (ZnS). ZnS nanowires were conjugated with different log dilutions of mercaptoacetic acid (MAA) and digoxin antibody (Ab). To observe the effect of each dilution on fluorescence of NWs, pH of the media was monitored before conjugation. This study highlights the enhancement in PL of ZnS NWs when pH of the media is almost equal to the pKa of carboxyl group of MAA (pH ~ pKa). While, quenching in fluorescence was observed when pKa < pH> pKa. Thus, proving our hypothesis of use of ZnS as a fluorescent tag for biological applications. The effect of pH, ions in media and time of incubation on the PL of MAA coated QDs was studied by incubating dots in three different phosphate buffers. The pH of media for each buffer was adjusted as acidic (pH <7.2), neutral (7.2 - 8.0) and basic (pH> 14.0).The PL response from dots in buffer 1 was more profound as compared to other two buffers. The current study demonstrated maximum fluorescence from dots in buffer 1 within pH range of 7.0 – 10.0, which increases with increasing time of incubation.Electrochemical analysis was performed to study the mechanism of electron transfer from QDs to linker (MAA) and then to Ab using electrochemical analysis. Electrochemical analysis shows resistance in electron transfer when electrode was modified with QDs and Ab conjugated QDs (QD-MAA-Ab) as compared to when modified with MAA only. The number of electrons calculated during the oxidation or anodic cycle were: 2, 1,3 and 4 for GCE, QD, QD-MAA and QD-Ab, respectively. During the reduction or cathodic cycle, the electrons found were 2, 1,4 and 6 for GCE, QD, QD-MAA and QD-Ab. Finally, detection of different log dilutions of digoxin inclusive of the therapeutic range (0.8-2.0 ng/ml) spiked in PBS buffer (1ml) using emission (quantum dots (QD)) and plasmon based (gold nanoparticles (SERS)) methods. Limit of detection (LOD) achievable through emission-based methodology was 0.5 ng/ml while 0.4 ng/ml through plasmon resonance of gold nanoparticles. Then the emission-based detection was performed in fabricated microfluidic device using homemade optical detection setup. The fabricated device is of ((H x W x D) 2.7’’ x 0.98” x 0.005)) as compared to conventional digoxin detection machine (Architect i2000SR: (H x W x D) 48’’ x 61” x 49”). The current study envisages replacement of conventional methodologies with microfluidic device or chip using quantum dots and SERs based tags as compared to conventional enzyme-based labels. The said device would be cost effective, portable and can easily be carried to remote areas of country.