بھٹو آج تک لوگوں کے دلوں میں کیوں زندہ ہے ؟وجہ
یہ ایک تاریخی تصویر ہے ۔ایک بوڑھا شخص 18اکتوبر 2007ء کو محترمہ بے نظیر بھٹو شہید کے استقبال کے لیے کراچی ائیر پورٹ ننگے پائوں آ یا ۔اس نے وہاں موجود صحافیوں کو یہ تصویر دکھائی اور بتا یا کہ میں آج اس واقعے کو دہرانا چاہتا ہوں جب میں اس طرح شہید بھٹو سے ملنے ننگے پائوں گیا تھا کیونکہ میرے اس وقت پہننے کے لیے جوتے نہیں تھے ۔شہید بھٹو نے جب میری یہ حالت دیکھی تو انہوں نے مجھے اپنے گلے سے لگایا ۔میں نے ان کی آنکھوں میں بغور دیکھا تو وہ بھیگ چکی تھیں ۔انہوں نے مجھے کافی پیسے بھی اور جب میں واپس جائے لگا توشہید بھٹو اپنے جوتے اتار کے خود بھی ننگے پائوں میرے ساتھ کا فی دور تک چلے !
Rotifers are zooplanktons that react more sharply to the environmental changes. Objective: To better understand the community composition of rotifers in Safari Zoo Lake, Lahore, PakistanMethods: wecollected 16 water samples on monthly basis, dividing lake into four major sites: northern, southern, eastern, and western sites. Each of these was further subdivided into four locations, for a total of 16 sampling sites. A total 23 rotifer species were recorded from the lake with Brachionus calyciflorus having maximum abundance. A species abundance curve was plotted between months and number of species of rotifers indicating their lowest and highest abundance through the study period. Results: A Cluster Analysis yielded three main groups of closely related species including species like Brachionus quadridentatus, Synchaeta stylata and Brachionus calyciflorusetc. We used a Principal Component Analysis (I and II) to study variation in the rotifer community on a seasonal basis and among sampling sites. A biplot of Principal Component Analysis (PCA I) reflected the relationship of rotifers with the months. Some of the species showed a positive positive relationship, while others showed negative one. PCA II was plotted between months and physico-chemical parameters showing their negative and positive relations. Conclusions: We conclude that a lesser replacement of fresh water, increased density of waterfowl, reptilia and solid waste left overduring boating has resulted in eutrophication of lake leading to overall low diversity of rotifers observed
Analysis of the genome sequence of Pyrobaculum calidifontis revealed the presence of an open reading frame Pcal_1127 annotated as ribose-5-phosphate pyrophosphokinase. To examine the properties of Pcal_1127 the coding gene was cloned, expressed in Escherichia coli, and the purified gene product was characterized. Pcal_1127 exhibited higher activity when ATP was replaced by dATP as pyrophosphate donor. Phosphate and EDTA activated the enzyme activity and equivalent amount of activity was detected with ATP and dATP in their presence. Recombinant Pcal_1127 could utilize all the four nucleotides as pyrophosphate donors with a marked preference for ATP. Optimum temperature and pH for the enzyme activity were 55 °C and 10.5, respectively. A unique feature of Pcal_1127 was its stability against temperature as well as denaturants. Pcal_1127 exhibited more than 95 % residual activity after heating for 4 h at 90 °C and a half-life of 15 min in the boiling water. The enzyme activity was not affected by the presence of 8 M urea or 4 M guanidinium chloride. Pcal_1127 was a highly efficient enzyme with a catalytic efficiency of 5183 mM−1 s−1. These features make Pcal_1127, a novel and unique ribose-5-phosphate pyrophosphokinase.