بال جبریل کی اشاعت کے چند ماہ بعد جولائی 1936ء میں ضرب کلیم منظر عام پر آئی۔ اقبال کا یہ اردو کا تیسرا مجموعہ کلام ہے” ضرب کلیم “ فکر کی پختہ اور مستحکم صورت کا مجموعہ ہے۔اقبال نے اس کتاب کے ہر حصے کو عنوانات میں تقسیم کیا ہے۔ عنوانات درج ذیل ہیں ۔ (1) اسلام اور مسلمان (ii) تعلیم و تربیت (iii) عورت (iv) ادبیات ، فنون لطیفہ (۷) سیاسیات مشرق و مغرب (vi) محراب گل افغان کے افکار۔ ہر عنوان میں بہت سی نظمیں ضمنی موضوعات کے تحت دی گئی ہیں۔ محراب گل افغان کے اذکار میں نمبر شمار دے کر بیس افکار پیش کیے گئے ہیں۔
Great lineage, good manners, trustworthiness, sincerity, courage and bravery, obedience, generosity, modesty, humility, thankfulness, absolute trust in Allah, education and upbringing, affection and compassion towards the elderly, the youth, slaves and animals, the regard for truth, the endurance and patience etc. Are such features that at them neither was the match for the Holy Master (saw) born, nor will any such person be ever born. In addition, let alone friends, his enemies were helpless to hold him as-Sadiq (the Truthful) and al-Ameen (the Trustworthy) due to these and many other great attributes; and they would say: Muhammad is not a liar at all, but we can’t believe in him as a prophet sent by Allah. Moreover, even a non-Muslim or polytheist has never criticized his character to this day, nor will anybody do till the judgment day. Whenever the greatest personalities of the world were listed or somebody committed to writing on the said topic, the Holy Prophet (saw) remained at the top of the list. Drawing of the swords of father and son against each other, the change of the standard of friendship and enmity, fighting of master and his slave against each other, chanting the slogan of Ahad-Ahad (the only One, the only One) on the blazing sand, giving priority to martyrdom over the utterance of a word of unbelief, taking a husband away from his wife and separating a mother from her infant, deforming the condition after a blasphemous blow on the blessed face, forced migration to Habsha (Abyssinia) at times, and laying siege to Shb-e-Abi Talib (the valley of Abi Talib) at times.
This study was conducted at Poultry Research Centre University of Agriculture Faisalabad, Pakistan to investigate the effect of olive, black and flax seed oils on growth performance and immune response of broiler chicks. The experiment comprised of three trials and there were three hundred birds in 1st and 2nd trial while in 3rd trial there were 150 birds. Day old broiler chicks were procured and were grouped into thirty experimental units having ten chicks each. Three levels (0.5, 1.0 and 1.5%) of olive, black and flax seed oils were offered having three replicates in each level. One group containing three replicates was serving as control which was fed commercial feed without supplementation of experimental oils. During first trial data on body weight, feed intake was collected weekly to calculate feed conversion ratio. Blood samples of three birds from each replicate were collected at the end of experiment to estimate the serum triglycerides, serum cholesterol, low density lipoprotein and high density lipoprotein. Three birds from each replicate were slaughtered to measure organs (liver, gizzard, heart, lungs, kidneys and spleen) and glands weights (pancreas, bursa of fabricious, thymus, adrenal and pituitary). Dressing percentage was calculated. Serum samples were taken to estimate titre against ND and IBD at 32nd and 35th day, respectively. During the first trail overall weight gain was significantly (P<0.05) highest in the treatment having flax seed oil 0.5%. Significantly (P<0.05) better overall FCR was observed in the treatment having olive oil 1.0%. For second trial, 300 day old chicks were divided into 30 experimental units having ten chicks each and were allocated the treatments in similar pattern as in first trial. During second trial humoral response and cell mediated immune response were assessed. Antibody titres against Newcastle and Infectious bursal diseases were observed weekly. Overall FCR was best in the group having flax seed oil 0.5%. Significantly highest value of total anti sheep red blood cells antibody titre of broilers fed various levels of different oils was recorded at 7th day post-secondary injection in the treatment having black seed oil 1.0%. Significantly highest value of immunoglobulin m anti sheep red blood cells antibody titre of broilers fed various levels of different oils was recorded at 7th day post-secondary injection in the treatment having black seed oil 1.0 %. Significantly highest value of IgM was recorded at 14th day post-secondary injection in the treatment having flax seed oil 0.5%. Significantly highest value of immunoglobulin g anti sheep red blood cells antibody titre of broilers fed various levels of different oils was recorded at 7th day post-secondary injection in the treatment having black seed oil 0.5%. During third trial best levels of oils from previous trials and blend of three oils (Having 3 levels 0.5%, 1.0% and 1.5%) along with control having no experimental oil in the feed. For this purpose, 210 day old chicks were purchased and were divided into 21 experimental units having ten chicks each. Data on weekly body weight, feed consumption was recorded to calculate feed conversion ratio. Intestinal pH, weight, length and histomorphology was recorded. Fatty acid profile and sensory evaluation of meat was also conducted during 3rd trial. Overall highest significant FCR during the third trial was recorded in the group having treatment blend 0.5%. Significantly highest value of intestinal weight was observed in black seed oil is 0.5%. Significantly highest level of oleic acid was found in the meat samples from the group having treatment blend 1.5%. Significantly highest level of linoleic acid was found in the meat from the group having treatment black seed oil 0.5%. Best overall FCR was observed significantly (P<0.05) in the treatment having olive oil 1.0%. Statistical analysis of the experimental data showed significant (P<0.05) effect on Newcastle Disease Titre due to varying levels of different oils in the feed of broiler birds. ND titre was significantly best in the treatment of olive oil 1.0% and flax seed oil 1.0% during 1st week. ND titre was significantly best in the treatments olive oil 1.0%&1.5% and flax seed oil 1.5% during 2nd week. ND titre was significantly best under the treatments of olive oil 0.5%, black seed oil 0.5%&1.0% during 5th week. IBD titer was significantly highest in the treatments olive oil 0.5%&1.5% during 5th week. Significantly highest value of total anti sheep red blood cells antibody titre of broilers fed various levels of different oils was recorded at 7th day post-secondary injection in the treatment having black seed oil 1.0%. Significantly highest value of immunoglobulin m anti sheep red blood cells antibody titre of broilers fed various levels of different oils was recorded at 7th day post-secondary injection in the treatment having black seed oil 1.0 %. Significantly highest value of IgM was recorded at 14th day post-secondary injection in the treatment having flax seed oil 0.5%. Significantly highest value of immunoglobulin g anti sheep red blood cells antibody titre of broilers fed various levels of different oils was recorded at 7th day post-secondary injection in the treatment having black seed oil 0.5%. Overall highest significant FCR during the third trial was recorded in the group having treatment blend 0.5%. Significantly highest value of intestinal weight was observed in black seed oil 0.5%. Significantly highest level of oleic acid was found in the meat samples from the group having treatment blend 1.5%. Significantly highest level of linoleic acid was found in the meat from the group having treatment black seed oil 0.5%. Overall flaxseed oil 0.5% was more economical. Data collected was subjected to statistical analysis using analysis of variance technique under Completely Randomized design. Treatment means were compared by Least Significant Difference test. Economics for each treatment was calculated.