ڈاکٹر اعجاز حسین ؍ ڈاکٹر مسیح الزماں
گزشتہ مہینہ میں ڈاکٹر اعجاز حسین سابق صدر شعبہ اردو الہ آباد یونیورسٹی کا انتقال حرکت قلب بند ہوجانے سے مظفرپور میں ہوگیا، جہاں وہ ممتحن بن کر گئے ہوئے تھے، ان کی میت الہ آباد لائی گئی، اپنی ملازمت سے سبکدوش ہونے کے بعد بھی لکھنے پڑھنے کا شغل جاری رکھا تھا، اہم اور مفید کتابوں کے مصنف تھے، جن میں مختصر تاریخ ادب اردو اور نئے ادبی رجحانات وغیرہ زیادہ مقبول ہوئیں، اپنے شاگردوں میں بہت مقبول رہے، ان کی وفات سے اردو ادب ایک بہت ہی لائق مصنف اور خدمت گزار سے محروم ہوگیا، ان سے کچھ ہی روز پہلے ڈاکٹر مسیح الزماں ریڈیو شعبہ اردو الہ آباد یونیورسٹی کی بھی وفات اچانک ہوگئی، اردو کی مرثیہ نگاری ان کا خاص موضوع تھا، ان کی عمر وفا کرتی تو اس صنف میں ان کا ادبی کارنامہ بڑا قابل قدر ہوتا، وہ پروفیسر مسعود حسن رضوی سابق صدر شعبہ اردو لکھنؤ یونیورسٹی کے داماد تھے، جن کے لئے اس کبرسنی میں یہ سانحہ بڑا ہی جانکاہ ہوگا۔
دعا ہے کہ خدا اردو ادب کے ان دونوں خدمت گزاروں کو غریق رحمت کرے، آمین ثم آمین۔ (صباح الدین عبدالرحمن، مارچ ۱۹۷۵ء)
Head of SMA Negeri 3 Palu implements the form of management based on five management functions, namely planning, organizing, staffing (placement), coordinating and controlling (control). In managing ecology-oriented schools refers to the school adiwiyata ie schools that maintain the environment, the arrangement of a clean environment, beautiful and healthy in order to produce a good learning environment. This effort also aims to provide understanding of the school community in the preservation of environmental functions and resources in the school environment. Environmentally sound environmental (ecological) school programs have participatory and sustainable principles. The participatory principle is that the school community involved in school management covers the whole process of planning, implementation, and evaluation based on responsibility. The principle of sustainability is that all activities are carried out comprehensively, planned and continuously in improving the learning conditions more comfortable and conducive for all citizens of the school.
Influenza viruses, in seasonal and pandemic forms, cause the most severe respiratory infections in humans. The currently licensed, egg-based vaccines fail to meet the global vaccine demand during a pandemic. Moreover, there is a constant need to reformulate these vaccines on annual basis according to the circulating viral strains. In order to develop efficient prophylactic measures, alternate vaccine strategies and production platforms need to be exploited. Subunit vaccines, like recombinant hemagglutinin, offer an alternative over conventional vaccines. Current study was designed to clone and express soluble influenza hemagglutinin (HA) of H9 and/or H7, which could be used as a vaccine candidate. To achieve high level of secretory expression and stabilized native trimer structure, HA was assembled with sequence for the tissue plasminogen activator signal peptide (tPA-SP) and foldon domain of bacteriophage T4 fibritin (FT) respectively by employing splicing by overlap extension amplification. The amplified product was cloned in pCMVR-8kb having human cytomegalovirus (CMV) immediate-early enhancer and promoter for transient expression of HA in 293F cells. SDS PAGE analysis confirmed a band of ≃ 70 kDa corresponding to H9HA and H7HA which was further confirmed by Western blot analysis and purified by Ni-NTA agarose column. The purified recombinant protein was found biologically active. This expression and purification process can be scaled up easily and recombinant protein can be produced rapidly which can then be used for further studies on virus-host interactions, viral pathogenesis and development of vaccines.The failure of currently available antiviral agents, due to high mutation rate of the influenza virus, calls for employing rigorous strategies to develop safe and potent inhibitory strategies against the virus. Nanoparticles have been studied extensively as potential antimicrobials, their applications recently extended to development of antivirals. In the present study, silver nanoparticles were successfully developed by green synthetic approach using bark extract of Cinnamomum cassia (Cinnamon) and leaves extract of Thymus vulgaris (Thyme). The synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, scanning electron microscopy (SEM) and fourier transform infrared spectroscopy (FTIR). Plant extracts (Cinnamon and Thyme) and their corresponding nanoparticles were tested against avian influenza virus subtype H7N3 in Vero cells and the viability of cells was determined by tetrazolium dye (MTT) assay. The silver nanoparticles derived from plant extracts enhanced the antiviral activity and were found to be effective in both treatments, when incubated with the virus prior to infection and introduced to cells after infection. Moreover, the safety profile of the extract and the nanoparticles showed that they were non-toxic to Vero cells even at a concentration of 500 μg/ml. The biosynthesized nanoparticles may, hence, be a promising approach to provide treatment against influenza virus infections. Further research on characterization and mechanism of action of nanoparticles is required to develop better antiviral therapeutics for highly mutating viruses.