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GLI3 gene is a well-known transcriptional repressor, majorly involved in developing the digits. As it is the promising gene for the digit development so recently many research is in process for exposure of potential application of this gene. The rapid increase of polydactylism in humans has led to more interest on the study of this gene from researcher and scholars. In this study we had taken the blood samples of 50 individuals including different families were collected, age ranges of the patients were from 3-75 years, and had symptoms from moderate to severe from different areas of District Bahawalnagar. Physical sings were used as the selection criteria. 3.5-4ml venous blood was drawn and stored into 50ml tube containing 400?l EDTA, labeled with code number. The results obtained through different characterization e.g. (inorganic method, DNA estimation, sequencing of PCR product and electrophoresis) for GLI3 gene exon 14. Thermo-cycler programs for the amplification of primers were not show any mutation in premier exons. Gel was visualized and in trans-illuminator and hence to ensure the amplified product. No further mutation has been observed in DNA strand. The DNA strands shows similar behavior of GLI3 exon 14 for effected and normal individual. The mutation in this specified GLI3 gene is not responsible for polydactylism. Therefore, GLI3 gene is not accountable for any mutation caused polydactylism in an individual.
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