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In different organs of the fish a few heavy metals were measured in some fishes Cirrhiunus reba, Channa marulius and Rita rita captured at Minchanabad Town in River Sutlej. The water and sediment samples were collected and analysed for metal concentration. With the help of pure Nitric acid all the samples were digested and only for gills perchloric acid (4:1) was used at 100 and reading was taken through spectrophotometer. The blood samples were collected from live fish through cardiac puncture and caudal vein. To check the effects of heavy metals, the haematological parameters were also used. The damage of DNA was evaluated through micronucleus test of blood erythrocytes. Similarly physiochemical parameters of water were also tested to check the level of nitrates, dissolved gases and pH results revealed that temperature recorded was 14.18 ? 2.09 ?C during winter and 24.15 ? 6.04?C in summer season. The average pH value was 8.01 ? 0.29 and 7.59 ? 0.49 during winter and summer seasons respectively. Total hardness was 115.20?10.43 mg/L in winter and 33.34?26.45 mg/L during summer. Dissolved Oxygen was 7.52 ? 1.04 and 7.15 ? 0.90 during winter and summer season. Mean concentration of heavy metals were significantly higher in winter season. Accumulation of Ni in sediments of centre of river was 32.4?0.65 ?g/g and 35.49?0.64 ?g/g along bank of the river. Level of Fe was 1847?2.65 ?g/g in main stream while 1818.3?2.52 ?g/g near bank. The Cd concentration was 0.0023?0.001 ?g g-1 in centre and 0.0018?0.001 ?g/g along banks. Copper were 54.37?0.82 ?g/g in main stream sediments and 35.18?1.75 ?g/g in sediments of banks. Chromium was 47.27?0.86 ?g/g in centreand37.1?1.06 ?g/g near bank in winter season. Lead was accumulated significantly higher in sediments with concentration of 93.11?0.16 in centre and 75.06?0.09 ?g/g along banks of river. A total of three fish species (Cirrhinus reba,Channa marulius and Rita rita) were also evaluated for bioaccumulation level of heavy metal concentrations Mean concentrations and standard deviations were applied for results for samples of during winter and summer season which were different statistically in all test organs and also in fish species.
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