Asian Research Thesis Index

Abstract

Pectinase recognized as an industrial important enzymes which occupies 10 percent in
enzyme production of world while 25 percent of food production. While keeping in
view in the light of above scenario the study was design to produce pectinase from
local agro wastes. The Pomelo (Citrus Grandis) was considered as novel substrate for
pectinase production through solid state fermentation by Aspergillus niger. In first
portion of this study, the OFAT (one factor at a time) was opted for optimizing the
conditions of temperature, pH and time period for pectinase production. While the
other factor was optimized thorugh RSM-DOE (Respone Surface Methadology), a
statistical tool for the analysis of variant factor on pectinase activity. About 14 RSM
trial were conducted by employing following condition i.e. Substrate (3-28g),
inoculum level (1.5-7.5ml) nitrogen (0.625-1.375g), moisture (5-105%). The highest
activity of pectinase was observed at pH (5), temperature (30 ?C), incubation day (4
)
by OFAT. In RSM maximum activity at 279.2 U/mL were achieved at optimize
temperature 30 ?C, pH 5, day 4
th
, moisture level 30%, inoculum size 6ml, substrate 3g
and nitrogen level at 0.250g. For purification, ammonium sulphate, dialysis and gel
chramatograpghy was performed. The results indicates that at 60% ammounium
sulphate precipitation was achieved with specific enzyme activity at 0.856U/mg
recorded after purification. Present finding proved that Pomelo peel was abundant and
cheap source for production at commercial.

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