Home
Add
Get on Google Play
Home
> Edit
Add/Update Thesis
Title*
Author's Name*
Supervisor's Name
Abstract
Dyslipidemia is an abnormal amount of lipids (e.g. triglycerides, cholesterol and/or fat phospholipids) in the blood. It is classified as presentation in the body (together with the specific type of lipid that is increased and underlying cause for the ailment (genetic, or secondary to another condition). Low density lipoprotein receptor gene is known as most important in the development of dyslipidemia. In this study, blood from patients (n=25) and normal individuals (n=25) were used to estimate lipid profile and DNA extraction. Lipid profiling was done through spectrophotometer technique. DNA extracted using standard organic method. DNA quantification will be done on agarose gel electrophoresis and nano-drop. Tetra-primers were designed for rs6511720 polymorphism in the LDLR gene to genotype through ARMS PCR technique. Designed ARMS PCR primers will be useful run on agarose gel to identify the genotype. Genotyping data was analyzed using statistical technique. 389bp was achieved in all of the samples which was control band and will be used to produce allele specific bands i.e. of 257bp and 192bp. Though the intensity of control fragments was weak, the samples are correctly genotyped as evident from the sequencing and BLAST results. Statistical analysis has shown the conformance with HW-principle showing that the samples were collected randomly. But association was not found because of low sample size. T-ARMS may be proven as rapid test to diagnose the mutation in LDLR gene causing the early prognosis of the dyslipidemia
Subject/Specialization
Language
Program
Faculty/Department's Name
Institute Name
Univeristy Type
Public
Private
Campus (if any)
Institute Affiliation Inforamtion (if any)
City where institute is located
Province
Country
Degree Starting Year
Degree Completion Year
Year of Viva Voce Exam
Thesis Completion Year
Thesis Status
Completed
Incomplete
Number of Pages
Urdu Keywords
English Keywords
Link
Select Category
Religious Studies
Social Sciences & Humanities
Science
Technology
Any other inforamtion you want to share such as Table of Contents, Conclusion.
Your email address*