Home
Add
Get on Google Play
Home
> Edit
Add/Update Thesis
Title*
Author's Name*
Supervisor's Name
Abstract
Climate change is a change in the statistical distribution of weather patterns when that change lasts for an extended period. Climate change and agriculture are interrelated processes and effect in number of ways. Pakistan has long spells of drought which, experts believe, are a result of climate change. Citrus fruits are one of the largest fruit crops in the world. In Pakistan ample availability of natural resources provides the favorable regime for the development of citrus industry. Citrus problem faces several problems including biotic and abiotic stresses. Role of rootstocks in citrus is one of the most debatable and discussed issues and its selection is a major consideration under planning any citrus orchard. Present research was planned for genetic characterization and evaluation of modern rootstocks tolerant/resistant to selected abiotic stresses i.e., drought and high temperature. Experiments were conducted to screen out various modern citrus rootstocks including local commercial rootstocks (Rough lemon and Sour orange) against high temperature and water stress. Drought was induced based on field capacity of citrus rootstock relative to water requirement of citrus, high temperature stress was applied with relative to five years maximum temperature range. Four-month-old uniform seedlings were selected for the stress treatments. Gas exchange parameters were recorded at four different times. Horticultural parameters, plant water status and chlorophyll fluorescence at different wavelength was recorded of stresses. From overall results it was concluded that Brazilian sour orange and Keen sour orange showed tolerance against selected stresses and Savage citrange and Yuma citrange were susceptible against the stresses. For the confirmation of tolerance, expression of stress related genes was tested. Proper detailed bioinformatic analysis were performed for the selection of primer related to heat shock protein and major intrinsic proteins. One tolerant (Brazilian sour orange) and one susceptible (Savage citrange) was selected for the genetic expression analysis against the heat shock proteins (CsHsp70, CsHsp70.1 and CsHsp90) and major intrinsic proteins (CsPIP2, CsTIP2 and CsTIP1). Genetic expression of genes showed Brazilian sour orange with high expression level of Hsp70 and Hsp90 against the temperature stress. MIP such as P1P and T1P depicted high expression against the water stress and both Hsp and MIP expression under combined drought and temperature stress. It can be concluded from the study that Brazilian sour orange was tolerant against high temperature and water stress, but Savage orange was susceptible.
Subject/Specialization
Language
Program
Faculty/Department's Name
Institute Name
Univeristy Type
Public
Private
Campus (if any)
Institute Affiliation Inforamtion (if any)
City where institute is located
Province
Country
Degree Starting Year
Degree Completion Year
Year of Viva Voce Exam
Thesis Completion Year
Thesis Status
Completed
Incomplete
Number of Pages
Urdu Keywords
English Keywords
Link
Select Category
Religious Studies
Social Sciences & Humanities
Science
Technology
Any other inforamtion you want to share such as Table of Contents, Conclusion.
Your email address*