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Karnal bunt disease caused by (Tilletia indica) is sporadic in nature. The fungus attacks the wheat crop in Feb. and March which are the susceptible stages (booting stage) of the host. During the survey it was estimated the disease is extending from north to south Punjab that are hot and dry areas of the province. Epidemiological studies revealed that range of minimum air temperature between (11-16 oC), maximum air temperature (27-31 oC) and rainfall more than (1.15 mm) during the vulnerable growth stages produced the critical circumstances for the invasion of the fungus on the wheat crop. Based upon the environmental conditions of two years data combined stepwise regression indicated that all the employed environmental factors explained 84 % of the in disease development, Out of 200 germplasm lines/ varieties only 16 lines were highly resistant and 9 were resistant. Protective spray of fungicides (Dolomite and Shelter) reduced the disease 62 to 63 % respectively.
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