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The genus Cleome L. is systematically revised from Pakistan and the genus is represented by 11 species including one new species. The genus Cleome is distributed in all four provinces of Pakistan, grown wild as well as cultivated. Globally it is found in tropical sub-tropical and warm regions of the world. The data from various aspects i.e., general morphology, seed and leaf micromorphology, palynology and chemistry have been obtained and analyzed numerically and distribution pattern of the genus is also studied. The genus Cleome is generally characterized by the presence of complete and tertramerous flowers with parietal placentation, two valved capsule with persistent replum, grooved seeds, tricolporate and more or less trilobed pollen grains, presence of carbon and oxygen in seed coats and quercetin and its derivatives in leaves. While, the genus Cleome can be mainly divided into two groups usually based on simple and compound leaves and each of the species shows great diversity by having distinct combination of pollen tectum and colpal membrane surface. Similarly, a variety of trichomes like pubescent, hirsute or scabrous is also observed. Artificial keys are constructed based on studied aspects and the data is also analyzed numerically by clustering to find out the relationship among the species of the genus Cleome from Pakistan.
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