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Our study consists of three parts. Part -1 deals with epidemiological study in which one year field survey of 2400 thoroughbred horses at Sahiwal, Lahore, Sargodha and Mandi Baha-ud-Din districts were surveyed revealing the prevalence of strongylosis as 40.83 %, 38.17 %, 43.33 %, and 39.83 % respectively. This study has drawn a compelling evidence that the disease is prevalent thoroughout Punjab province with remarkable overall prevalence of 40.54 %. We further explored that hot and humid environment favours its prevalence, age and sex of the horses has no effect on the prevalence and it is maximum in summer while lowest in winter. This study also demonstrates 79.58 % prevalence of internal parasitism among thoroughbred horses overall at Punjab province. Part -2 deals with study of haematology, serum enzymes and electrolytes on 30 thoroughbred horses suffering from large strongyles infection and treated with piperazine adipate. This study revealed a higly significant (p >0.05) decrease in RBC, Hb, Neutrophil percentage, Na, K and Mg in diseased horses. The decrease in RBCs and haemoglobin exhibited normocytic normochromic anaemia. There was a significant decrease in monocyte percentage in diseased animals. A highly significant (p >0.05) increase in eosinophils, SGPT, SGOT, LDH, and a significant increse in basophils percentage was recorded in diseased horses. No significant increase / decrease (p <0.05) was noted in the levels of TLC, lymphocyte percentage, CPK and Ca in diseased horses. Part 3 deals with therapeutic trials of allopathic and herbal drugs. This study was conducted on 110 thoroughbred horses suffering from large strongyles infestation. Present study revealed that aqueous extract of N. sativa seeds at the dose rates of 50, 100 and 150 mg/kg body weight is highly effective and showed 87.50 %, 87.82 % and 88.35 % efficacy 28 days post treatment respectively. Aqueous extracts of F.parviflora arial parts at the dose rates of 50, 100 and 150 mg/kg body weight showed 62.74 %, 80.68 % and 81.74 % efficacy 28 days post treatment respectively. Aqueous extracts of Fl. macrophylla arial parts at the dose rates of 50, 100 and 150 mg/kg body weight exhibited 66.92 %, 73.38 % and 74.04 % efficacy 28 days post treatment respectively whereas 100 % efficacy rate of piperzine adipate (allopathic drug) at the dose rate of 110 mg/kg body was noted. Among the herbal drugs efficacy was found maximum in N.sativa followed by F.parviflora whereas the lowest with Fl. macrophylla.
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