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Hypericum oblongifolium is a flowering plant in Hypericaceae family. It is found at an altitude of 4000-6000 meter especially, in Himalaya, China and northern parts of Pakistan. It has been traditionally used in Chinese herbal medicine for treatment of bacterial and viral infection, burns, hepatitis, swellings, bruises, nasal hemorrhage and inflammations. A series of pharmacological properties, ranging from wound healing and antiseptic to antiviral, anti-inflammatory, anticancer, ethanol intake inhibition and apoptosis-inducing activities have been associated with this plant. This plant has been proved to be act as anti-ulcer, anti-proliferative and anti-inflammatory agent. The plants also contain compounds which are chymotrypsin, urease and lipoxygenase inhibitors. H. oblongifolium have also been reported for its antispasmodic, bronchodilator, hypotensive and anti-myocardial infraction behavior. In the present study whole plant of H. oblongifolium was selected for the identification and isolation of medicinally important natural products present in it. From the chloroform and ethyl acetate soluble fractions of H. oblongifolium, sixteen compounds have been isolated. Four compounds were considered as new natural products, while twelve compounds were identified as known compounds. Their structures were elucidated by the use of sophisticated modern spectroscopic techniques (1H-NMR, 13C-NMR, HMBC, HMQC, 1H-1H- COSY, NOESY, HREIMS and HR-FAB-MS) and several chemical tests. Following are the structures of new compounds.
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