Home
Add
Get on Google Play
Home
> Edit
Add/Update Thesis
Title*
Author's Name*
Supervisor's Name
Abstract
MicroRNAs (miRNAs) are short, endogenous and non-protein coding RNAs that are 18-26 nucleotides (nt) in length. The main function of the miRNAs is to negatively control the protein coding sequences at post-transcriptional level. By nature majority of the miRNAs are evolutionarily conserved. This conserved nature provides the logic to predict the new miRNAs orthologus in a number of plant species. In plants, miRNAs are involved in almost all biological processes from growth and development to biotic and abiotic stresses with metabolism, apoptosis and cell signaling. In the current dissertation, various computational tools were used for the profiling and characterization of new conserved miRNAs and their targets in four important vegetables of Solanaceae viz: tomato (Solanum lycopersicum L.), potato (Solanum tuberosum L.), eggplant (Solanum melongena L.) and chilli (Capsicum annuum L.). Consequently, a total of 393 miRNAs (belonging to 364 miRNA families) were discovered from the mining of 7,63,564 express sequence tags (ESTs). Of which, 120 miRNAs (110 miRNA families) were from potato, 109 miRNAs (106 miRNA families) from tomato, 88 miRNAs (81 miRNA families) from chilli and 76 miRNAs (67 miRNA families) were from eggplant. In this study, a total of three miRNA genes were found as pre-miRNA clusters, where, two belong to chilli (can-mir-8019 and can-mir-8036) and one is from potato (stu-mir-6164). One potato miRNA family stu-mir-8123 was also found both as an antisense (mir8123a) and sense (mir8123b). A total of thirty randomly selected miRNAs were subjected to reverse transcription-PCR (RT-PCR) validation studies. Furthermore, a total of 1592 protein targets were also predicted for these newly profiled miRNAs. These predicted protein targets were classified as: transcription factor, stress related, disease related, growth and development, hypothetical protein, transporters, signaling pathways, metabolism and structural protein. All of these newly identified miRNAs were reported in tomato, potato, eggplant and chilli for the first time. These results will serve as reference data to improve the regulation, management, and modification of these economically important crops at the molecular level. This will also help us to improve solanum plants for production and biotic and abiotic stress tolerance in the near future.
Subject/Specialization
Language
Program
Faculty/Department's Name
Institute Name
Univeristy Type
Public
Private
Campus (if any)
Institute Affiliation Inforamtion (if any)
City where institute is located
Province
Country
Degree Starting Year
Degree Completion Year
Year of Viva Voce Exam
Thesis Completion Year
Thesis Status
Completed
Incomplete
Number of Pages
Urdu Keywords
English Keywords
Link
Select Category
Religious Studies
Social Sciences & Humanities
Science
Technology
Any other inforamtion you want to share such as Table of Contents, Conclusion.
Your email address*