Home
Add
Get on Google Play
Home
> Edit
Add/Update Thesis
Title*
Author's Name*
Supervisor's Name
Abstract
Background: Reliable distinction between AML and ALL is important for the selection of appropriate therapy. The final diagnosis of acute leukemia should be based on clinico-pathological, morphological, cytochemical and molecular cytogenetics data, together with immunophenotypic information. Flow cytometry has become the preferred method for the lineage assignment and maturational analysis of malignant cells in acute leukemias. Immunophenotyping has also allowed the detection of aberrant antigen co expression and the analysis of heterogeneity and clonality of malignant cells in acute leukemias. It is insufficient to merely make a diagnosis of acute leukemia, or even AML or ALL. Further classification is essential in order to determine the prognosis and select the most appropriate treatment. Furthermore, immunophenotypic characteristics of acute leukemias have not been described in Kenya yet and the existing immunophenotypic patterns of the disease in Nairobi are not known. Objective: The main objective was to determine the relative frequencies of Acute Leukemia immunophenotypes using commonly expressed markers and to describe clinicopathological characteristics. Setting: The study was carried out at Aga Khan University Hospital Laboratory between March 2009 and March 2010. Patients and methods: One hundred and thirty two consecutive blood and bone marrow specimens were analyzed for cytomorphological characteristics and immunophenotypic markers. The clinical-pathological characteristics were also recorded for each patient with acute leukemia. The immunological category was assigned using the EGIL criteria. The end point was the immunophenotypic profiles and the association of each immunophenotype with the clinical and pathological features. Immunophenotyping was done by Flow cytometry. Results: There were 132 patients in total in whom immunophenotyping was done. These comprised of 67(50.8%) males and 65(49.2%) females with M: F ratio of 1.03:1. The age ranged from 2 years to 87 years with a median age of 29.5 years. There were 88 cases of AML and 42 cases of ALL. Only 2 cases of biphenotypic leukemia were studied. AML was found to occur more in adults with a total of 76 (86.4%) cases in patients above 15 years of age and only 12 (13.6%) cases in those below 15 years of age. The commonest AML immunophenotype in all patients was AML-M2 accounting for 26 (29.5%) cases with 19 (21.5%) cases seen in adults and 7(7.95%) in children. The peak adult age range for AML patients was 41-50 years. The proportion of AML-M4 in this study was 19.3%. Majority of ALL cases were B-ALL phenotype at 29 (96.6%) with the dominance
Subject/Specialization
Language
Program
Faculty/Department's Name
Institute Name
Univeristy Type
Public
Private
Campus (if any)
Institute Affiliation Inforamtion (if any)
City where institute is located
Province
Country
Degree Starting Year
Degree Completion Year
Year of Viva Voce Exam
Thesis Completion Year
Thesis Status
Completed
Incomplete
Number of Pages
Urdu Keywords
English Keywords
Link
Select Category
Religious Studies
Social Sciences & Humanities
Science
Technology
Any other inforamtion you want to share such as Table of Contents, Conclusion.
Your email address*