Liver is an essential organ that controls around various physiological functions such as production of bile, plasma proteins, and hormones aids in digestion and detoxification of drugs as well. Drug induced liver injury (DILI) is the principal cause of acute liver failure (ALF) in various countries. There is a broad array of anti-psychotic drugs (APDs) which are used to treat bipolar disorders most commonly schizophrenia and other neuro-psychotic disorders. One of the two famous anti-psychotic agents; aripiprazole and ziprasidone belong to atypical class of anti-psychotics. Although APDs are currently being used all over the world yet these drugs have displayed many side effects. In-vivo estimation of acute liver injury level by APDs therefore bears significance so as to determine the efficacy and identify potential health hazards. Current study will determine the liver injury induction potential of APDs in animal model.To induce acute liver injury, acetaminophen (APAP) was used. The foremost drug responsible for DILI is APAP which induces oxidative stress and dysfunction of mitochondria. Collection of blood samples was done in aseptic conditions, and collected serum was subjected to biochemical estimations. Serum ALP and ALT levels has been assessed to determine the effect of APDs on liver functioning efficiency. Estimation of serum ferritin has provided iron storage estimation. Moreover, the study had also evaluated the renal efficiency changes induced by APDs via estimation of urea and creatinine levels. HDL, LDL, TG and cholesterol levels were also estimated to identify the effect of these drugs on lipid profile. Thus, the study will provide a rationale for the utilization of APDs and their effects on a body system, in comparison to APAP induced acute liver injury.