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Home > Association Analysis of Ldl-Cholesterol With Celsr2, Apoe and Apob Genes Through Arms-Pcr

Association Analysis of Ldl-Cholesterol With Celsr2, Apoe and Apob Genes Through Arms-Pcr

Thesis Info

Access Option

External Link

Author

Umair Zubair

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=235

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721002394

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Dyslipidemia is term to demonstrate the abnormal lipid metabolism that may lead to cardiovascular diseases.Several variants have been already reported to be associated with abnormal lipid metabolism. Four different variants CELSR2 (rs629301), APOE (rs429358 & rs7412) and APOB (rs1367117) were analyzed by Tetra Primer-Amplification Refractory Mutation System Polymerase Chain Reaction (T-ARMS PCR).This technique uses an approach that in addition to amplifying the control fragment (which surrounds the allele of interest), the mismatch, at the point of SNP on allele, on 3? terminus in additional inner primers makes these primers specific to one allele and refractory to other one. An additional mismatch near the 3? terminus of the inner primers strengthen the allele specificity. An amplicon is produced or not produced, in this condition, thus demonstrate the genotype of the subject. Though the optimization and development of T-ARMS PCR was difficult and time consuming but the method allows to genotype the specific variant and was validated by Sanger Sequencing method. As with other techniques, this technique has also its advantages and disadvantages.Variants having high GC content cannot be genotyped because of having high melting temperature and therefore formation of stable secondary structures thereby halting the amplification procedure even when organic additives were added. Therefore T-ARMS assay could not be developed for APOE (rs429358 & rs7412) variants. The second part of the study was to find the association analysis of the selected variants. T-ARMS PCR assay was developed for the CELSR2 (rs629301) and APOB (rs1367117) variants. Individuals having normal and abnormal lipid profiles (n=25 each) were examined for association analysis. Genotype distribution were found to be in Hardy-Weinberg Equilibrium and Chi square test indicates the significant association for both variants. Large scale genotyping approach can be employed for other along with these variants by using plate-based heat-block on PCR machine. As T-ARMS PCR Technique is robust and cost-effective, early risk detection can save the time window of treatment and would ultimately enhance the life expectancy of the patients suffering from dyslipidemia.
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ہویا جثہ ڈانواں ڈول

ہویا جثہ ڈانواں ڈول
مینوں اندروں ویکھ پھرول
میرے منہ وچ تیرا ناں
کردے مینوں لوک مخول
سن کے خبر مہنگائیاں والی
دل وچ ہندا ہردم ھول
گلاں رج کے کر نہ سکے
بھانویں رہندے کول کول
کوئی بھانویں ویکھے نہ ویکھے
ڈر کے رب توں پورا تول
موت سرے تے ہر دم بندیا
اُچا بول نہ ہر گز بول
اگاں وچ نہ ساڑ گلاباں
مٹی وچ نہ موتی رول

Dehumanization of Refugees: A Postcolonial Eco-Critical Study of Mohsin Hamid’s Exit West

This paper explores Mohsin Hamid’s literary representation of dehumanization of refugees in the age of globalization, the exploitation of humans and their land, as well as the impact of migration on the lives of the migrants in his novel Exit West. The research uses the theoretical framework of postcolonial ecocriticism by Huggan and Tiffin to critically analyze the primary text. This research employs qualitative method to draw parallels between the exploitation of humans and their environment in Hamid’s novel while exploring the dehumanized treatment of refugees, reasons of migration, and the role of war, science and technology in the destruction of environment in this particular narrative. Textual analysis, more specifically, content analysis is used as a method to link the global humanitarian and environmental crisis in the primary text under the Postcolonial Eco-critical ideology.

Prevalence of H9 Avian Influenza Virus Among Poultry Workers in District Rawalpindi, Pakistan

Avian influenza virus (AIV) subtype H9 carries great zoonotic potential and human infections have been reported in influenza endemic countries. To the best of our knowledge, very little is known of seroprevalence of H9 in human population in Pakistan. The main intention with which the current study conducted to report the prevalence of the H9 virus among the poultry workers and to add a new piece of knowledge to its prevalence in Pakistan which is the first step taken for before control measures can be executed.Overall 325 sera samples were obtained from poultry encounterd population and a general publicfrom January 2018 to September 2018 in Rawalpindi, Pakistan. Haemagglutinin-inhibiting (HI) assay was performed to determine the seropositivity for serum samples. Overall seroprevalence level of anti-H9 antibody titres was 12 % (39/325) in occupational individuals and general population (control group). Anti-H9 antibody titres was 8.8%, 8.% and 34.1% in age groups of 16-39, 40-59 and >60 years respectively of poultry-exposed workers. On the other hand, anti-H9 antibody titres of general population was 25% and 16.6% in age groups of 16-39 and 40-59 respectively. Interestingly, anti-H9 antibody titres in the general population among 16?39 was 25% and among 40?59 was 16.6%. The highest percentage of sero-positivity (13.7%) of antibody titer to H9N2 was found in the poultry butchers followed by poultry farm workers 11.4%. The lowest seropositivity (7.1%) was recorded in veterinarian. Serum samples from males revealed significantly higher anti-H9 antibody titre (12.4%) than females (8.5%).It revealed that antibody titer of butcher, had significant (P<0.05) difference from antibody titer of poultry salesmen and veterinarians but non-significant difference from poultry farm workers. Antibody titer of butcher had differed significantly (P<0.05) as compared to group of general population. In the control group (general population group), 17 individual (68%) had no detectable antibody titer against H9N2 and only 12% people had antibody titer of = 1:40. To recapitulate, our findings revealed poential transmission AIV H9 subtype from avian to humans and highlighted that poultry professional linked people are at great risk of AIV infections than general population. Hence, further detailed and more systemic approaches to study seroprevalence ofH9N2 AIV infections in human and live poultry population in all over Pakistan is recommended to determine the relations between clinical significance and seropisitivity in humnas and identify possible solutions to reduce the transmission of AIV in poultry workerfrom live poultry.