حیرت ہے، مے کدہ ہے کہ جنت خیال ہے
اک خوش نما سراب میں بھٹکا غزال ہے
دیکھے جو تجھ کو، دیکھ کے پھر ہوش میں رہے
باطل ہے اس کا دعویٰ، بڑا کج خیال ہے
قربت نے تیری بخش دیے فاصلے مزید
مجھ کو، مرے نصیب کو، سب کو ملال ہے
آنکھوں سے تم پکارو، ادھر وقت سے بھی تیز
میں دوڑتی نہ آئوں مری کیا مجال ہے
یاد اس کی ہے فضاؔ مری سانسوں میں دم بہ دم
رک جائے اک گھڑی بھی تو جینا محال ہے
Law plays a pivotal role in the establishment of any peaceful society. Islam, being proactive, devised important rules about 1400 years back for the safety of Deen, life, wealth, wisdom and Generation. Qatal (murder) is a crime of taking soul of a humanbeing, about which Islam has announced Qisas i.e to do with assissinater what he has done it to killed human being. In the same manner Pakistan penal Code has gathered rules about crimes steped out in Pakistan. So Pakistan penal code, under several sections has the same punishment. This artcle throws light on Pakistan penal code sections about death Senctance in perspective of Islamic imperium, order and explanation.
Fungal endophytes colonize an important niche within the plants through secretion of secondary metabolites. The metabolites and extracellular enzymes produced by endophytic fungi regulate the growth of the host plant and contribute in defence mechanisms.The medicinal plants Caralluma acutangula and Boswellia sacra were used for the isolation of endophytic fungi. The endophytic fungi were identified as Penicillium citrinum, Paecilomyces variotii, Aspergillus nidulans, Fusarium oxysporum, Epicucum nigram, Penicillium purpurogenum, Penicillium spinulosum, Aspergillus caespitosus, Phoma and Alternaria sp. and were assessed for their potential to produce anti-cancerous metabolites by performing MTT assay and extracellular enzymes such as cellulases, phosphatases and glucosidases in growth media. P. variotii, P. citrinum and F. oxysporum showed significantly higher amount of phosphatases and glucosidases as compared to other strains. Additionally, P. variotii and F. oxysporum showed significantly higher potential of indole acetic acid production (tryptophan-dependent and independent pathways). ACC (1-Aminocyclopropane-1-carboxylate) deaminase results showed that P.citrinum, P. purpurogenum and P. Variotii had shown prominent ACC deaminase activity (300 nmol α- ketobutyrate mg-1h-1). Fluorescence-based MUB (4-methyl umbelliferone) standards were used to analyze the presence of extracellular enzymes glucosidase, phosphatase and cellulase. The bioactive secondary metabolites from endophytic P. citrinum also revealed some prominent results by performing MTT assay on breast cancer cell line (MCF-7). The current study concludes that these fungi are producing bioactive constituents that could provide unique niche of ecological adaptation by symbiosis and greatly contributing to the healthy life of their host plant. However, some of the endophytic fungi offer a great potential to produce anti-cancerous metabolites and extracellular enzymes.