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Antimicrobial Susceptibility and Esbl Prevalence of Clinical Pathogenic Pseudomonas Species

Thesis Info

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Author

Manzoor Ahmad

Program

PhD

Institute

Hazara University

City

Mansehra

Province

KPK

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Biochemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10004/1/22-07-2018%20PHD%20Thesis%20for%20hard%20binding.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725513609

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The current study was conducted from 2010 to 2014 in Khyber Teaching Hospital, Peshawar, Pakistan to determine the Susceptibility of Pseudomonas spp. to different chemotherapeutic agents and prevalence of extended spectrum β-lactamase. The samples were taken from three main tertiary care hospitals of Peshawar, Pakistan. During the study period, 3450 specimens including pus, urine, blood and burns etc. were collected and subjected to culture and sensitivity as per standard protocols. Samples were isolated and identified on the basis of standard biochemical techniques. Antimicrobial susceptibility was determined by modified Kirby-Bauer method and Minimum Inhibitory Concentrations were followed per the guidelines set by CLSI. The ratio of male to female under the study was 1:1.4. The most productive antimicrobial agent was class carbapenem (imipenem and meronem) against Pseudomonas spp. among the β-lactam agents whose susceptibility was 282 (84.43%) and 304 (91.02%) respectively. The resistance rate was highest for Tetracycline followed by Penicillin and the isolates were co-resistant to macrolides and flouroquinolones and a moderate activity was demonstrated to the Cephalosporins. A total of 232 isolates were recovered from hospitalized and 102 from OPD patients. Statistically significant values were obtained for 17 out of 20 antibiotics, as there is a remarkable variation in susceptibility pattern of OPD and Hospitalized isolates. In class carbapenems: imipenem and meronem showed 81.47%; 91.18% and 88.79%; 96.08% activity rate for indoor and outdoor strains respectively. Only tetracycline had a diminished rate for both in and out door (5.17% and 21.57%) isolates respectively. In-patient isolates showed higher rates of resistance to most tested antibiotics, compared with outpatient isolates. Overall, there was a moderate decrease in susceptibility rate of Pseudomonas spp. to the antibiotic analyzed over the last five years of the study. The MIC50 & 90 (μg/ml) of imipenem against Pseudomonas spp. was <1 and < 4, respectively. While results obtained by agar dilution method demonstrated the lowest MICs values with meropenem for Pseudomonas spp. MICs observed for carbapenems as compared to the other antimicrobials tested were higher. xix Initial screening and phenotypic confirmatory test for ESBL detection was carried out according to the CLSI protocols. Production of ESBLs was observed in 148 (44.32%) of the isolates and the remaining 186 (55.80%) were non-ESBL producers. Statistically, a significant difference was found in susceptibility of the Pseudomonas spp. to carpenems, quinolones and β-lactam/β-lacatamase inhibitors, among the ESBL producers. The resistance conferred by ESBL’s producing pseudomonas spp. to cephalosporin’s (CEC, CAZ, CRO and CFP) was 14.2%, 20.3%, 14.3% and 22.3% respectively, contrary to the non–ESBL’s, where they were comparably sensitive to 3rd and 4th generation cephalosporins. Treatment with third generation cephalosporin (ceftriaxone) is the only risk factor being associated with ESBL infections. β-lactamases of these strains analyzed genotypically by PCR with a series of primers specific for tem, shv and ctx-M genes. 100 samples were selected for PCR to detect tem, shv and ctx-M genes among the ESBL positive Pseudomonas spp. strains. A high proportion of isolates were confirmed for ctx-M gene which encodes a total of 48 strains followed by tem 38 and then 14 of them were shv genes.
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الإمام زفر: أصوله الفقهية وآرائه المفتي بها في تفسير الأحكام

Compilation and codification of Islamic jurisprudence is a great achievement of the Islamic literary history. The productivity andfertility of the mind of the Muslims Scholars was a true and brilliant field of cognitive process. Ilanafi school of thought display in these Muslims societies from practice point of view always dominated. In this respect the Imam Zufar (RA) (110AH) had been a great scholar in Ilanafi Canon with his unique multi-dimensional features and characteristics. Nevertheless, He iras a prominent jurist, Muhaddis, Justice and Mujtahid. He paved a different path to have difference of opinion with his great teacher Imam Abu Hanifa as well as with the rest offamous jurists. This article emphasis on those principles along with examples that at the end evolved to have become his exclusive principles for inference of the Shari. ahka am. This article will also highlight his versatile scholary life and will help the researchers to investigate about his seminal work.

Occurrence of Aflatoxin in Groundnut and Spices During Storage

Aflatoxins are secondary metabolites produced by the species of Aspergillus, specifically Aspergillus flavus and Aspergillus parasiticus. The aflatoxins are highly toxic, mutagenic, teratogenic and carcinogenic compounds implicating as causative agents in human hepatic and extra hepatic carcinogenesis. The present project was divided into three parts. In the first part (Study I), the chemical changes in spices and groundnuts were studied under different packaging materials and storage conditions. There was no aflatoxin was detected in all groundnut varieties and spices. Cumin, red chillies and black pepper samples stored in jute bags showed maximum moisture contents as compared to high density polyethylene (HDPE) bags. Moisture content in cumin samples also increased significantly at 85% RH. Moisture content in all the samples increased as a result of storage and humidity. The storage, humidity and packaging materials showed non-significant effect on ash, protein, fat, fiber, nitrogen free extract (NFE) and total phenolics in groundnut varieties. The total fungal count, Aspergillus count and total aflatoxin in all spices was significantly (p< 0.01) affected due to packaging material, relative humidity, storage intervals and interactions of these variables. However, low level of total aflatoxin was detected in spices as compared to groundnuts. The chemical constituents like crude protein, crude protein, crude fiber, ash and NFE differed significantly among different groundnut varieties. However, storage intervals, packaging materials and interactions between these variables non-significantly affected the proximate composition of groundnuts varieties except moisture content which was increased during storage. In study II, after 120 days of storage the contaminated samples were packed in HDPE and irradiated by gamma radiation @ 2, 4 and 6 kGy and stored at room temperature and relative humidity in paper carton box along with control (0 kGy) for 90 days. The gamma radiation showed significant effect on total fungal count and aspergillus count as compared to control. Irradiation and storage showed non-significant effect on proximate composition and total phenolics of spices. The third (Study III) comprised the use of groundnut oil in cookies as to replace normal shortening and its effect on quality attributes of cookies during two month of storage period. The physico-chemical analyses and fatty acid profile of groundnut oil was performed. The results regarding fatty acids profile of groundnut oil indicated that GNO contain low level of saturated fatty acids; myristic acid (0.04%), palmitic acid (9.85), stearic acid (2.53), arachidic acid (1.21), behenic acid (2.52) and lignoceric acid (2.42%) while higher level of un-saturated fatty acids oleic acid (55.36%), linoleic acid (26.96%) and Eicosenoic acid (1.36%.). The groundnut oil was used in cookies preparation @ 25, 50, 75 and 100 % along with control (0% groundnut oil) and packed in HDPE bags for 2 months of storage at room temperature. The cookies were evaluated for physical, chemical, sensory and storage stability analyses. The moisture content, crude protein and Thiobarbituric acid (TBA) no. of cookies significantly varied as a function of storage, where as crude fiber, ash and NFE content of cookies varied non-significantly. The width, thickness and spread factor of cookies varied significantly due to variation of groundnut oil in cookies. The results of the present investigations indicated that spices and groundnuts can be safely stored in polyethylene bags for their better quality retention. The use of radiation can be helpful for the preservation of spices and groundnuts with respect to the production of aflatoxin during storage.