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Home > Biodiversity in Pears Pyrus Spp. : Characterization and Conservation of Germplasm from Azad Jammu and Kashmir

Biodiversity in Pears Pyrus Spp. : Characterization and Conservation of Germplasm from Azad Jammu and Kashmir

Thesis Info

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Author

Maqsood Ahmed

Program

PhD

Institute

Bahauddin Zakariya University

City

Multan

Province

KPK

Country

Pakistan

Thesis Completing Year

2008

Thesis Completion Status

Completed

Subject

Applied Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/handle/123456789/257

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676725606399

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A survey of five pear growing districts of Azad Jammu and Kashmir i.e. Rawalakot, Bagh, Muzaffarabad, Sudhnoti and Kotli was conducted during the year 2003 and 60 accessions of distinct characters of horticultural importance were selected from 48 sites of these districts for characterization. For each accession, passport data were collected with the help of Pyrus descriptor as developed by International Board of Plant Genetic Resources. During the next two years (2004 and 2005), all the selected accessions were visited thrice each year at the time of flowering, fruit setting and fruit maturity. Data were recorded on growth habit of plants, incidence of diseases (fire blight and apple scab), precocity, intensity of flowering, fruit setting, productivity, time of ripening, fruit shape and fruit colour. Leaves and fruit of these accessions were also collected and leaf area, fruit size and fruit weight were measured. Ripe fruit were evaluated for organoleptic parameters, nutritional value (TSS, total sugars and vitamin C content) and postharvest life at ordinary room temperature (26 ± 2 oC). The accessions differed for these parameters; however, the accessions with the same local name had almost similar characteristics. All the accessions locally called as Frashishi and Desi nashpati had the excellent fruit quality and can be exploited for commercial production in the area. Most of the other accessions, also had good fruit quality except few (locally called as Btangi, Btung, Raj btung and Pathar nakh), which are mostly used as rootstocks in the area. The accessions were characterized by using protein markers based on bio-chemical analysis (SDS-PAGE). The accessions differed in number of bands which ranged from 12- 20. According to the banding pattern the accessions were divided into the various groups and sub-groups showing similarities and differences among them. Out of sixty local xxaccessions, fifty six accessions along with eight varieties (used as reference control) were also characterized using DNA based SSR markers to assess genetic diversity and relationship among them. Nine out of 12 primers revealed clear and reproducible amplification banding pattern in 41 genotypes (33 accessions and 8 control varieties). Cluster analysis based on UPGMA dendrogram, grouped the genotypes into clusters sub- clusters and groups on the basis of relatedness and variability. Most of the accessions were absolutely homogenous and were classified into two homogenous groups, despite the fact that these accessions differed in there morphological and physico-chemical traits. Attempts were also made to preserve the local pear germplasm through in vivo and in vitro methods. For in vivo preservation, a nursery was established and nine pear genotypes i.e. Khurolli, Bagugisha, Pathar nakh, Desi nakh, Kotharnul, Desi nash, Frashishi, Kashmiri nakh and Raj btung were propagated through whip grafting on Btangi seedlings. The genotypes differed for growth parameters. Apical shoots of nine pear genotypes (already mentioned above except Desi nash but including Btangi) were preserved in vitro under minimal growth conditions using low temperature treatments, modification in medium strength and adding mannitol in the basal medium and also by reducing the concentration of BAP and using growth retardants for different storage periods i.e. 3, 6, 9 and 12 months. The genotype differed significantly for survival and regeneration percentages. Storage at 5 °C, MS medium containing 2.5% mannitol and MS medium with reduced concentration of BAP (0.50 mg l -1 ) were found significantly better as compared to other treatments. Storage for the minimum period (3 months) was better than other storage periods as assessed on the basis of survival and regenerability of the cultures.
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جینے کے ڈھنگ تیری جدائی سکھا گئی

جینے کے ڈھنگ تیری جدائی سکھا گئی
اور یاد تیری مجھ کو ہے شاعر بنا گئی

آندھی سفر کی رات میں جو چل پڑی تو کیا
اچھا ہوا کہ راہ کے پتھر ہٹا گئی

دورِ جنوں میں رہتا تھا خوش باش میں بہت
یہ آگہی تو میری ہنسی کو ہی کھا گئی

فرقت کی رات نیند ہمیں آتی کس طرح
ہم کو شبیہ یار تھی کہ جو سلا گئی

دیکھو اداسی بھی کسی گربہ سے کم نہیں
چھوڑا کسی جگہ بھی تو پھر گھر کو آ گئی

اپنا یہاں ہے کون پرایا ہے فہدؔ کون
یہ راز ہم کو ایک مصیبت بتا گئی

عقد نکاح میں کفاءت کی حقیقت

Kafa'at is a term used in the field of Islamic jurisprudence which means equivalence and closeness between the expected spouse i.e. Male and female in specific area. So it may be defined as the compatibility or equivalence between the expected husband and his expected wife which have to be adhered to in future. This compatibility standard suggested by Islam is highly effective, that include religion, profession, wealth, lineage, moral and social standard. The Hanafi, the Shaft’s and the Hanbali schools concur in requiring kafa'at in religion (Islam), freedom (i.e. In his not being a slave), profession and lineage. These schools differ regarding kafa'at in wealth. The Hanafi and the Hanbali schools recognize it, while the Shaft’s school does not. The Maliki School do not accept the notion of kafa'at except in religion. The emphases of this Kafa'at are to make the spouse relation pleasant, everlasting and coherences. The alikeness suggested by Islam between male and female is also traditional and logical value which Islam highly encouraged. The hidden philosophy of Kafa'at is to build the relation between the expected spouses more effective and pleasant.

Characterization and Application of Dye Decolorizing Metal Tolerant Bacterial Community for Treatment of Textile Wastewater

Bioremediation of colored textile wastewater loaded with dyes and metals is a matter of great concern due to the hazardous risks associated with their untreated discharge into water bodies. The present study was conducted to isolate and characterize dye decolorizing metal tolerant bacterial strains for treatment of textile wastewater. For this purpose, 220 metal tolerant bacteria isolated from textile wastewaters coming from different textile industries in Faisalabad were tested for their potential to decolorize four different reactive dyes viz. reactive red 120 (RR120), reactive black 5 (RB5), reactive orange 16 (RO16) and reactive yellow 2 (RY2). Based on the potential to decolorize RR120, RB5, RO16 and RY2 in the presence of a mixture of four heavy metals (Cr, Cd, Zn, Pb), four bacterial strains viz. ZM17, ZM130, ZM160 and ZM183 were selected for subsequent studies. These strain were identified following the amplification, sequencing and bioinformatics analyses of 22 their 16SrRNA, and were named as Raoultella sp. ZM17, Pseudomonas aeruginosa strain ZM130, Pseudomonas sp. ZM160 and Pseudomonas sp. ZM183. The selected bacterial strains were found to have varying levels of tolerance against the presence of different heavy metal ions in the media. Moreover, the decolorizing potential of these isolates was found to be decreased with an increase in the initial dye concentration. Furthermore, the interactive effects of four input variables including salt content, pH, C source concentration and level of multi-metal mixture on decolorization of RB5 by the selected bacterial strains were examined following response surface methodology (RSM) statistical modeling. All the four strains were also found capable of simultaneously removing Cr(VI) and all the reactive azo-dyes under study. However, the strain ZM130 was found to be the most efficient among these strains. Additionally, these strains also showed considerable potential for production of IAA and phosphate solubilization. The strains ZM17 and ZM183 were also found to harbor a dye decolorizing azoreductase Azr gene, whereas, a cadmium tolerant czcABC gene was found to be amplified from the DNA of the strains ZM130, ZM160 and ZM183. Enzymatic analyses of the strain ZM130 indicated that azo-reductase and tyrosinase activities were absent in this strain, however, this strain exhibited considerable activity of laccase and NADH-DCIP reductase activity during the decolorization of RR120. While testing the applicability of the strain ZM130 for treatment of synthetic wastewater, efficiency for color and COD removal of this strain was found to be favored and better in the anaerobic batch bioreactor as compared to aerobic batch bioreactor system. However, presence of yeast extract significantly increased the potential of ZM130 for color and COD removal with attached growth of microbial culture. The potential of the most efficient dye decolorizing metal tolerant bacterial strain Pseudomonas aeruginosa ZM130 for treatment of synthetic colored wastewater containing both RB5 (300 mg L-1) and hexavalent chromium (25 mg L-1) was also tested in vertical soil columns bio-augmented with this strain using sterilized and nonsterilized soil. It was interesting to note that the highest level of color, COD and Cr(VI) removal (˃90%) was achieved in the treatments where the soil columns were bio-augmented either with the sludge alone or the sludge along with ZM130 followed by the strain ZM130 along with addition of yeast extract. Based on the findings of the present study, it might be concluded that the selected bacterial strains, particularly Pseudomonas aeruginosa strain ZM130, harbor a varying but highly considerable potential for bioremediation of different xenobiotic compounds including dyes and heavy metals. Furthermore, the concurrent plant growth promoting ability offers a great promise for increased and sustained crop productivity even under stressed soil environment. Hence, this strain could be used to develop the bio-treatment systems (bioreactors) for addressing the problem of azodyes and metals in the wastewater.