Search or add a thesis

Advanced Search (Beta)
Home > Evaluation of Biological Potential of Quercus Dilatata L. and Isolation of Active Compounds

Evaluation of Biological Potential of Quercus Dilatata L. and Isolation of Active Compounds

Thesis Info

Access Option

External Link

Author

Madiha Ahmed

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Pharmacy

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/11259/1/Madiha%20Ahmed_Pharmacocognosy_2018_QAU_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726114555

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Natural products have been the mainstay in treating debilitating and multipronged diseases since the dawn of medicine. The current study was designed to isolate and characterize biologically active lead compounds from an underexplored medicinal folklore Quercus dilatata L. Total 42 extracts from each of the aerial parts, nut shells and galls were prepared using sonication aided maceration as the extraction procedure. The extract library was subjected to a range of phytochemical and in vitro bioassays in order to identify most functional plant part and extraction solvent for preparative extraction. Phytochemical investigation comprised of standard colorimetric assays to determine phenolic and flavonoid contents while, RP-HPLC was carried out to establish polyphenolic profile. MTT assay was employed to determine leishmanicidic activity against Leishmania tropica whereas disc diffusion assay was performed to elucidate antibacterial, antifungal and protein kinase inhibitory spectrum. Starch-iodine chromogenic assay determined the α-amylase inhibitory potential while brine shrimp lethality. MTT and SRB assays were used to find cytotoxic potential of the subject plant. Among all extracts, maximum gallic acid equivalent phenolics and quercetin equivalent flavonoids were quantified in distilled water-acetone aerial parts extract (21.37±0.21 μg GAE/mg DW) and methanol-ethyl acetate galls extract (5.28±0.30 µg QE/mg DW) respectively. RP-HPLC revealed the presence of substantial amount of various phenolics (from 0.049±0.01 to 15.336±1.55 μg/mg extract) including pyrocatechol, gallic acid, catechin, chlorogenic acid, pcoumaric acid, ferulic acid and quercetin in aerial parts extracts. Maximum reducing power potential and total antioxidant capacity was recorded in methanol-ethyl acetate and distilled water extract of galls i.e. 50.76±1.0 and 48.57±1.1 µg AAE/mg DW respectively. Highest free radical scavenging efficiency was exhibited by methanolethyl acetate aerial parts extract (IC50 8.1±0.5 µg/ml). A noteworthy leishmanicidic potential (IC50 12.91 μg/ml) was exhibited by the ethyl acetate-acetone aerial parts extract whereas, maximum antibacterial activity against Staphylococcus aureus (MIC 12.5 µg/ml) was manifested by ethyl acetate aerial parts extract. Substantial protein kinase (PK) inhibition (28±0.35 mm of bald zone of inhibition) was exhibited by methanol extract of aerial parts. Ethyl acetate galls extract showed 52.5±2.75% inhibition of α-amylase activity. Chloroform-methanol aerial parts extract showed maximum cytotoxicity against brine shrimp larvae with IC50 value of 34.54 μg/ml while significant cytotoxicity against THP-1 and Hep G2 cells was shown by nhexane and ethanol aerial part extracts with inhibition of 46.73±0.85% and 82.52±1.45% respectively. Keeping in view the abovementioned results, aerial part was selected for preparative extraction with chloroform-methanol (1:1) as its extraction solvent. Preparative extract (QDC) was partitioned through solvent-solvent extraction and the resulting fractions (QDN, QDE, QDB, QDA) were biologically evaluated to prospect fraction hits for lead development. Maximum α-amylase and leishmanisidic activities were revealed by QDE i.e. 52.44±3.21% and 83.0±2.35% respectively. QDN was found to possess maximum cytotoxic activities against MCF-7 (50.7±3.24%), MDA-MB 231 (40.0±2.31%) and Hep G2 (46.3±1.54%) cell lines whereas, QDB had the highest free radical scavenging potential (IC50 17.55 µg/ml). Maximum inhibition of TNF-α activated NF-κB (67.90±3.27%) and NO production in LPS-activated murine macrophage RAW 264.7 cells (84.90±2.01%) was shown by QDE. QDN was found to be most active in aromatase inhibition assay (61.11±3.76%) and quinone reductase 1 induction assay (induction ratio=7). On the basis of aforementioned results, QDN was selected as hit fraction for leads isolation and was subjected to normal phase gravity and medium pressure liquid column chromatography to yield 4 compounds (QDN2, QDN4, QDN5 and QDN9). Biological evaluation of compounds suggested that maximum α-amylase, PK and antipromastigote activities were presented in case of QDN5 i.e. 19.5±2.30%, 6.5±0.10 mm ZOI and 48.0±2.30% respectively. Likewise, QDN5 showed maximum inhibition against MCF-7 (54.2±1.54%), MDA-MB-231 (43.7±2.40%) and Hep G2 (45.2±3.50%) cancer cell lines. QDN5 showed maximum cancer chemopreventive proficiency via inhibition of NF-κB (65.42±9.70%) and NO production (78.0±1.10%) assays followed by QDN2 with 63.0±5.30 and 65.1±3.20% inhibition of NO production and NF-κB respectively. X-ray crystallographic and spectroscopic studies characterized the structure of QDN2, QDN4 QDN5 and QDN9 as friedelin, 3epifriedelinol, glutinol and taraxerol respectively. In principle, the results of the current study endorses Q. dilatata as a substantial source of bioactive lead compounds.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

حضرت خدیجہؓ

حضرت خدیجہ
خدیجۃ الکبریٰؓ کی شادیاں : حضرت خدیجہ ؓکے والد خویلد عرب کے مشہور تاجر تھے ۔ قریش میں نامور تھے ان کی وفات کے بعد خدیجہؓ نے ان کے تجارتی کاروبا ر کو سنبھالا دیا اور اسے وسعت دی ۔ عفت و پاک دامنی کے سبب خدیجہ طاہرہ کے لقب سے جانی پہچانی جاتی تھیں ۔ ان کی پہلی شادی عتیق بن عائذ مخزومی سے ہوئی ‘ ان میں سے دو اولادیں ہوئیں ۔ ایک لڑکا عبداللہ بن عتیق اور ایک لڑکی ہند بنت عتیق ۔ خدیجہ کے شوہر عتیق کا انتقال ہوا ۔ اس کے بعد خدیجہ کی دوسری شادی ابو ہالہ بن زرارہ تمیمی سے ہوئی ۔ ان سے ہالہ ‘ طاہراور ہند پیدا ہوئے ‘ یہ تینوں بھائی صحابی تھے ۔ ( رحمۃ اللعالمین ۔۱۴۴۔ ۲ ) وہ مزید لکھتے ہیں کہ بعض سیرت نگاروں نے خدیجہ کا پہلا نکاح ابو ہالہ بن زرارہ سے ہوا تھا ‘ لکھا ہے ‘ اس بات میں مورخین کا اختلاف ہے ۔ ’’ قتادہ ‘‘ نے عتیق کا پہلا نکاح بتایا ہے اور جرجانی نے ابو ہالہ کا ۔ صاحب الاسیتعاب نے بھی جرجانی کے قول کو صحیح کہا ہے ۔ میں (سلمان) نے قتادہ کے قول؛ کو اس لیے پسند کیا ہے کہ صاحب الا ستیعاب نے ہند کو ربیب رسول اللہ لکھا ہے اور یہ تب ہی ہو سکتا ہے کہ ابو ہالہ کے بعد نبی مکرم ﷺ کا نکاح ہوا ہو ( حوالہ بالا )
دونوں شوہروں کا انتقال ہوا ‘ بعد ازاں قریش کے سرداروں نے انہیں نکاح کرنے کے پیغامات بھیجے مگر خدیجہؓ نے سب پیغامات ٹھکرا دیئے لیکن آپ کے پیغام کو بہ دل و جان قبول کیا۔ نکاح کے پیغام کو قبول کرنے کی وجہ ابن اسحاق یوں بیان کرتے ہیں جو خود خدیجہؓ کے الفاظ میں یہ...

ﺣﻖ اﻟﺘﺸﺮﻳﻊ ﺑﻴﻦ اﻟﻨﻈﺎم اﻟﺪﻳﻤﻘﺮاﻃﻲ واﻹﺳﻼم

Legislation means enacting Laws and regulations that run people's lives. The democratic system gives this right to the members of Parliament and Senators, or Committees to frame laws for promoting the human right in this system. While the legislation in Islam vested in Allah Almighty, and not shared by any close angel or a Prophet. It is confined in the Holy Quran and Sunnah of Mohammad (SAW. Islamic system provides

Partitioning of Dry Matter and Nitrogen under Fertilizer-N Levels and Decapitation Stress in Wheat

Wheat (Triticum aestivum L.) if cut before boot stage can be used for both forage and grain purposes, however it needs better nutrient management along with other agronomic practices to reduce the possible loss in grain yield. Experiments were conducted on imposing decapitation stress on wheat in comparison to no cut under different N levels and its application techniques to assess the crop response. The decapitation stress was imposed through cutting of foliage at 60 d after sowing. Three levels of nitrogen i.e. 100, 150 and 200 kg ha-1 were applied as full dose at sowing (M1), full dose at 2nd irrigation (M2) and split (M3) as half at sowing and half with 2nd irrigation to cut (C1) or no cut (C0) wheat. Treatments were arranged in randomized complete block (RCB) design with four replications having a plot size of 3 x 5 m2. The dry matter yield along with N concentration of different plant parts were determined at different growth stages to assess the translocation of photosynthate and N at these various stages. Wheat cultivar Fakhre Sarhad was planted on 1st November during both growing seasons with seed rate of 120 kg ha-1. All plots applied with recommended levels of 50 kg P2O5 and K2O ha-1. The experiment was conducted on silty clay loam alkaline soil (pH = 7.7-8.2) at Agricultural Research Farm (Malakandher), NWFP Agricultural University Peshawar during 2004-05 and 2005-06. The experimental site was located at 17o, 35 ́ N and 35o, 41 ́ W with altitude of 450 meters above sea level. Decapitation of wheat crop at early growth stage provided sufficient fresh forage but on the cost of reduced spikes m-2, grains spike-1, plant height, thousand grains weight, biological and grain yields when compared to no cut plants. However, harvest index remained same for both treatments. Cut treatment of wheat enhanced the N content at boot and anthesis stages but not at maturity. While grain and straw N contents, grain protein and nitrogen harvest index (NHI) were the same for decapitated and un-decapitated plots. Cut plants had lower N uptakes, apparent nitrogen re-translocation and its efficiencies compared to un-cut plants. Nitrogen use efficiency was increased with decapitation compared to no cut plants. Increased nitrogen fertilization either in split or late application as full dose had resulted in high N content at boot stage, anthesis, and maturity stage. Increasing N levels up to certain point had increased straw and grain N content, nitrogen uptake, grain protein content, nitrogen harvest index, apparent N re-translocation efficiency were increased, whereas NUE was reduced with increasing the N levels. Late application of N as full dose or even two equal splits applications at sowing and/or with 2nd irrigation had improved the N content , total N uptake, N re- translocation and its efficiency, grain protein, and NUE. However, the application time had no effects on N content and protein contents at maturity as well as NHI. Nitrogen fertilization had improved soil total nitrogen after harvesting the wheat. Days to emergence and emergence m-2 was not affected with fertilizer-N and its levels. Delayed boot stage was observed with fertilizer-N and its split application. Nitrogen fertilization as well as split application had increased spikes m-2, plant height and thousand grains weight. Increased N fertilization and full application or two equal splits had increased the grains spike-1, grain and biological yields, and harvest index. The dry matter of wheat stem, leaves and flag leaf at boot stage enhanced with fertilizer-N. The fertilizer-N increased stem and flag leaf dry matter at boot stage with increasing fertilizer-N level where no impact on leaves dry matter was observed. The two equal split doses improved stem and leaves dry matter, delayed full dose improved leaves dry weight while fertilizer-N timing had no influence on flag leaf dry matter. The decapitation stress increased stem and flag leaf dry weight but reduced leaves dry weight at boot stage. The dry matter of stem, leaves, flag leaf, peduncle and spike improved with fertilizer-N at maturity compared to control. The increasing fertilizer-N levels had no influence on stem, leaves and flag leaf dry matter but enhanced peduncle and spike dry matter at maturity. Fertilizer-N timing had no impact on stem, spike, leaves and peduncle dry matter at maturity while only flag leaf dry matter improved with full delayed dose of N application. The decapitation stress reduced leaves, stem, flag leaf, peduncle and spike dry matter at maturity. On the basis of economic analysis optimum VCR (value cost return) and relative increase in income (RII) obtained with 150 kg N ha-1 split application. Where additional Rs. 4959 ha-1 as net income over control were obtained with decapitation of wheat crop. Keeping in view the results two equal split doses 150 kg N ha-1 at sowing and 2nd irrigation is recommended for higher grain yield and economic return.