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Home > Molecular Epidemiology, Virulence Potential and Source Tracking by Source Specific Markers of Campylobacter Jejuni

Molecular Epidemiology, Virulence Potential and Source Tracking by Source Specific Markers of Campylobacter Jejuni

Thesis Info

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Author

Siddiqui, Fariha Masood

Supervisor

Syed Habib Bukhari

Program

PhD

Institute

COMSATS University Islamabad

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Subject

Natural Sciences

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/7499/1/Fariha_Masood_Siddiqui_Biosciences_CIIT_2016.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726702943

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Globally Campylobacter jejuni, major cause of bacterial human gastroenteritis, is frequently isolated from poultry, cattle and waste water. The goal of this research was to assess (1) the isolation frequency of C. jejuni from diverse sources, (2) antibiotic resistance profiling, (3) source attribution and distribution in clusters, (4) occurrence of newly identified Type VI secretion system (T6SS). Samples were collected from Islamabad, Karachi, Lahore, Peshawar and Gilgit. Overall 1305 samples were collected from different sources, then confirmed by morphology, biochemical tests and PCR. Antibiotic susceptibility was determined by disc diffusion method according to CLSI 2010. Beta lactamase producing strains were identified by Nitrocefin method. C. jejuni isolates were grouped into clusters by two triplex predictive PCRs. Multiplex PCR based on T6SS conserved genes was employed to screen Pakistani C. jejuni isolates and their gene expression was determined by RT-PCR. C. jejuni strains were isolated from 13.6 % clinical, 46.47 % poultry, 21.40 % cattle and 22.06 % wastewater samples. Highest resistance rates were observed among poultry and wastewater isolates. Highest resistance was observed for nalidixic acid, erythromycin and ceftriaxone. Most sensitive antibiotics were found to be chloramphenicol and spectinomycin. Beta lactamase producing strains were detected in 35 % clinical, 57 % poultry, 39 % cattle and 32 % wastewater isolates. Group clustering showed majority of the isolates were in C4/C6 and C7/C8 groups while none were assigned to C1/C2/C3 cluster. Results of identification of T6SS in C. jejuni isolates revealed 4.64 % of all the isolates were positive for T6SS. Gene expression analysis demonstrated down-regulation of T6SS in acidic environment. Our results showed a high isolation frequency of multidrug resistant C. jejuni isolates in different sources. Source tracking PCR revealed majority of clinical isolates were tracked to nonlivestock sources. Surveillance strategies should be intended to lessen the burden of C. jejuni infections by limiting transmission from livestock as well as non-livestock sources. Our findings regarding T6SS highlight the need to establish the role of the T6SS in environmental survival or virulence.
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راکی ولسن کا’’مینار محبت‘‘

راکی ولسن کا’’مینار محبت‘‘

کائنات میں اشرف المخلوقات ہونے کا شرف صرف انسان کو حاصل ہے تمام مخلوقات میں انسان سب سے زیادہ با اختیار بھی ہے۔احساسات و جذبات بھی سب سے زیادہ انسان ہی رکھتا ہے۔اسی لیے وہ ازل سے اپنے خیالات کا اظہار کرتا آیا ہے۔کبھی آرٹ ،تصاویر،لوک داستانیں اور کبھی شعر وادب کی صورت زبان کا اظہار بہترین ذریعہ ہے۔لفظوں کو ایک خاص انداز میں اپنے خیالات کے اظہار لے لیے استعمال کرنا شاعری ہے۔راکی ولسن نے بھی اپنے خیالات کے اظہار کے لیے خوبصورت الفاظ کا استعمال کیا ہے۔اس لیے اسکی شاعری و خود جذبات کے تحت وجود میں آتی ہے۔

راکی نے رشتو ں ناتوں میں بٹے ہوئے انسان کو اس کی خوشیوں،غموں،امنگوں، آرزوؤں،اْمیدوں،نا امیدوں،رویوں اور ولولوں کو اپنا موضوع بنایا ہے۔اسکی بے ساختہ شاعری فطرت انسانی کی عکاس ہے،وہ موضوعاتی شاعری پر بھی طبع آزمائی کرتے دکھائی دیتے ہیں۔انکی شاعری میں متاثر کرنے کی زبردست صلاحیت موجود ہے۔یہ صلاحیت صدق دل اور خلوص نیت کے بغیر حاصل نہیں ہوتی۔یہ خلوص نیت ان کے مجموعہ کلام ’’مینار محبت‘‘ میں محبت بانٹتے ہوئے دکھائی دیتا ہے۔آپ کے اس مجموعہ کلام اجمالی جائزہ کچھ اس طرح سے ہے۔

اگرچہ شاعر نے شاعری کی روایت سے ہٹ کر اس کتا کو شروع کیا ہے۔مگر اس کے باوجود وہ اپنے پروردگار کو بھولے نہیں۔کہتے ہیں کہ ہر شے میں اس ذات باری تعالیٰ کا جلوہ موجود ہے اسی لیے تو کائنات کی ہر شے اس کی خوبصورتی کو دیکھنا چاہتی ہے،آج راکی صاحب جو کچھ بھی ہیں وہ سب اس ذات کی کرم نوازی کی وجہ سے ممکن ہوا ہے:

حسن فطرت کے مظہر ہے تیری شان وجیہہ

تیری دید کی طالب ہر آنکھ شیدائی ہے

Issues of Ethnic Diversity and Just Development in Pakistan with a Special Focus on the Seraiki Ethnic Group

Ethnicity implies the sense of belonging together as the cultural group in a given society. It is a complex combination of racial, cultural and historical characteristics by which people differentiate themselves from other groups. This research envisages the genesis and the evolution of ethnicity as a political concept, the problems of ethnicity in a heterogeneous, multicultural state and the phenomenon of ethno-nationalism in its historical and analytical perspective in the federation of Pakistan with special reference to the status of Seraiki ethnic group. In fact ethnic expressions exist in all multicultural states and distinct ethnic groups evaluate themselves through communal prism. The less privileged groups develop abhorrence against the over – privileged groups due to the persistence of socioeconomic injustices. Factors like the gap between core and periphery, asymmetrical modernization and authoritarian trends lead towards ethnic disruption. Same is the case with Pakistan, a multilingual, multiracial and multiethnic state with federating units reflecting various diversities. The analysis of ethno-nationalism in Pakistan highlights factors, like regional cultural identity, relative deprivation among regions, centralized state structure, denial of accepting regional language as national language, and the absence of democratic values as being the root causes of the Bengali separatism. The assimilationist policies of the government do not acknowledge the regional/ethnic aspirations. Denial of pluralistic approach has been thwarting the demand for provincial autonomy. The nature of ethnic consciousness in the Seraiki belt, analyzed in this article, is found to be nurtured by the perceived socio-economic injustice at intra-provincial level—between the regions of South Punjab and Central and Northern Punjab put together

Livestock Potentials in Balochistan: Prospects and Constraints With Special Reference to Small Ruminants

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