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Home > Phytochemical Investigation of Alnus Nitida and Computational Studies of Some Selected Natural Products

Phytochemical Investigation of Alnus Nitida and Computational Studies of Some Selected Natural Products

Thesis Info

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Author

Hameed, Rabia

Program

PhD

Institute

COMSATS University Islamabad

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Chemistry

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/10931/1/Rabia%20Hameed_Chem_2019_Comsats_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726923157

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Alnus nitida is a medicinally important plant native to western Himalayas. In Pakistan it is distributed in northern areas and found abundantly in Abbottabad and Mansehra districts. Owing to the importance of the plant as a folk medicine, its wide distribution in the area and the fact that very little phytochemical work has been carried out so far on this plant, male flowers of Alnus nitida were selected for our present phytochemical investigation. In this study, we have investigated nine compounds (all new isolates of Alnus nitida) including a new one: close chain diarylheptanoid (2). They have been completely characterized by using optical rotation, ECD, UV, IR, MS, and NMR techniques. Structure of compounds 2 was validated through theoretical studies as well. The new compound 2 as well as known compounds were subjected to anticancer activity (in vitro) against cervical cancer. For this specific site cancer evaluation, HeLa cell lines and BHK-21 cell lines were used. Results showed that these compounds are potent anticancer agents. Some of them showed much higher activity compared to the reference drug, carboplatin. Computational studies have made tremendous achievements in the recent past. Conformational flexibility of dipeptides and correct assignment of isomeric compounds are challenging tasks. In the current work, we have tried to address these challenges. In the first study, the effects of intramolecular basis set superposition error and dispersion in the determination of correct lowest energy conformer of tyrosine glycine dipeptide molecule, by employing different levels of theory (B3LYP, B3LYP-D3, MP2, and M062x), are explained. The study identified the use of the best density functionals for conformationally flexible compounds especially those having aromatic moiety. In the second study, 1H-1H and 13C-1H coupling constants of possible stereoisomers of quinic acid were predicted to provide reliable assignment of chirality and positions of the hydroxy groups for quinic acids isolated from plants or the synthesized quinic acid derivatives. Analogous DFT techniques can be used to study systems similar to quinic acid such as octulosonic acids etc. In conclusion, nine isolated compounds (triterpenoid, flavonoids, open chain diarylheptanoids and close chain diarylheptanoids) with potent anticancer activity as well as conformational analysis of Tyr-Gly dipeptide and distinction of stereoisomers on the basis of coupling constants is a contribution in the field of natural product and computational chemistry.
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جنرل ہوریس کارپینٹر

جنرل ہوریس کارپینٹر

            امریکہ کے نامور جنرل ہوریس کارپینٹر، جنھوں نے ابھی وفات پائی ہے، ان کی جائداد کی کل قیمت ۷۲۱۲۰۰ پونڈ (ایک کروڑ ۸۲ لاکھ روپیہ) نکلی، یہ ساری رقم ان کے وصیت نامہ کے مطابق تین امریکی یونیورسٹیوں میں تقسیم ہورہی ہے، نیویارک کے ایک اور متوفی رئیس مسٹر لینی، ۱۰ ہزار پونڈ (ڈیڑھ لاکھ روپیہ) کا ترکہ یوسٹن یونیورسٹی کے لیے چھوڑ گئے ہیں۔ (جنوری ۱۹۲۰ء)

 

جدید تفسیری ادب میں بنت الشاطی کی امتیازی خدمات

Āisha bint Abdu Al-Rahmān (1913-1998), better known by her pen nickname ‘Bint Ash-Shātī’, was one of the 20th-century Egyptian exegetes (mufassirīn) of the Qur'ān who implemented the methodological approach to the interpretation of the Qur'ān introduced by her teacher, mentor and husband Amīn alKhawlī, an eminent Egyptian scholar of the Qur'ān who contributed to the Qur'ānic sciences from several important aspects. Bint Ash-Shātī continued the legacy of her teacher and published a number of works related to the discipline of the Qur'ānic sciences such as al-Tafsīr al-Bayānī li al-Qur'ān alKarīm and al-I’jāz al- Bayānī li al- Qur'ān wa Masā’īl Ibn alAzraq. She made an attempt to examine all the previous Qur'ānic exegeses. Though Bint Ash-Shātī benefited from the classical tafsīr literature in her writings, she made several critiques on the classical tafsīr literature. Because of her scholarly approach, she became a famous Qur'ānic scholar in her life and even she was awarded the King Faisal Award,  the most prestigious award in the Muslim world, in 1993 for her intellectual contributions. This paper attempts to analyze the methodological approach adopted by Bint Ash-Shātī in her works on Qur'ānic studies.

The Genetic Basis of Salt Tolerance in Gossypium Hirsutum L.

Development of cotton varieties having good performance for salt-affected soils is one of the prime objectives of the cotton breeding institutions across the country. Thus, the present research work was carried out to find information about the presence of sufficient variability in cotton germplasm at seedling stage as well as at full crop stage. Considering the importance of evolution of salt tolerant varieties, the present study was planned with objectives: (a) to screen out available of cotton germplasm to determining their salt tolerance potential, (b) to Check out the genetical behaviour of F1 cotton genotypes under salt stress, and (c) To find out F1 cotton genotypes having high yield potential than existing cultivars. The cotton germplasm comprising of 50 genotypes was exposed to salinity stress of NaCl @10 and 20 dSm-1 at seedling stage. Large variations were exhibited by all the genotypes for root and shoot length, root and shoot fresh and dry weights. Moderate to high coefficient of variance and high heritability coupled with high genetic advance reflected that improvement in salinity tolerance at seedling stage is possible through selection. Selection index and cluster analysis resulted in 6 tolerant genotypes (NIAB-999, CIM-707, NIAB-78, MNH-93, CIM-446 and CIM-443) and 3 susceptible genotypes (CIM-499, NIAB-111 and S- 12). The line×tester mating design (using 6 tolerant genotypes as lines and 3 susceptible genotypes as tester) was employed to study the inheritance pattern of yield contributing traits (plant height, number of bolls per plant, individual boll weight and seed-cotton yield), fibre quality traits (GOT%, staple length, staple strength, fiber fineness) and ionic concentration traits (Na+, K+ and K+/Na+ ratio) of cotton genotypes exposed to normal (control level) and salinity stress level of NaCl @ 20 dSm-1. Analysis of variance indicated huge variation for all traits studied and it was found that salinity caused severe reduction in all traits except in Na+ and GOT%. High heritability estimated along with moderate to high values of genetic advance for all yield contributing traits suggested that selection may result in considerable improvement of seed-cotton yield under salinity stress. Plant height, number of bolls per plant, individual boll weight GOT%, staple length, staple strength, K+ and K+/Na+ ratio under salinity stress had shown highly significant correlation with seed-cotton yield per plant. Highest direct effects on seed-cotton yield were exhibited by number of bolls per plant and individual boll weight. Additive gene action was exhibited by plant height and number of bolls per plant, Na+, K+ and K+/Na+ ratio under salt stress environment while for individual boll weight, seed-cotton yield and staple length, mixed trend of additive and non-additive gene action were found under salt stress. It was also found that salt tolerant genotypes showed relatively less uptake of Na+ but high uptake of K+ and K+/Na+ ratio under salinity stress. The hybrids NIAB-999× CIM-499 and CIM-707× CIM-499 (on the basis of heterosis and combining ability estimates) were the top 2 hybrids having superior yield and increased salt tolerance under salt stress environment. Combining ability analysis suggested that these above 2 F1 genotypes need to be further progressed through pedigree method of selection.