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Population Based Case-Control Asthma Association Studies of Single Nucleotide Polymorphisms in Chromosome 17 Potential Genomic Regions

Thesis Info

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Author

Shahid, Mariam

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Molecular Biology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12835/1/Mariam%20Shahid_%20Molecular%20Bio_2015_UoPunjab_PRR.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676726965604

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Single nucleotide polymorphisms (SNPs) on 17q21 are known to be associated with asthma disease in multiple populations. This study was designed to know whether this region is associated with asthma in Lahore region population or not. A total of 200 asthma patients and 100 healthy controls were enrolled from different hospitals of Lahore, Pakistan. Twenty five SNPs from chromosomal region 17q21 were selected for analysis in cases and controls by single base extension method and capillary based genetic analyzers. Associations with asthma were analyzed using basic allelic model, genotypic model and results were adjusted for various cofactors by logistic regression analysis using PLINK v1.9. Pair wise Linkage disequilibrium among the SNPs was analyzed and LD map from healthy controls was constructed using Haploview software. Five of the SNPs (rs3816470, rs1011082, rs6503525 rs11078928 and rs1008723) are suggestive of quite significant association with asthma under genotypic model. Five of the remaining SNPs (rs2290400, rs12603332, rs4065275, rs12603332and rs3894194) showed significant associations in the presence of cofactors like gender, smoke exposure in early years and urban or rural environment. Haplotype “TTGTACC” for rs11078928, rs2290400, rs1008723, rs7219923, rs4065275, rs12603332 and rs4795405, appears to be a significant risk factor for predisposition to asthma. The association of genomic region 17q21 with asthma in Lahori population is reported in this study.
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ابوالفیض سحر

ابوالفیض سحر
اردو کے ایک اور اچھے شاعر و ادیب اور تحریک کے خاموش مگر سرگرم اور مخلص خدمت گزار جناب ابوالفیض سحر ۲۲؍ جون کی شب میں حرکت قلب بند ہوجانے سے وفات پاگئے اور ۲۳؍ جون کو ۱۱ بجے دن میں بستی حضرت نظام الدین میں واقع قبرستان میں دہلی کے شعرا و ادبا اور متعلقین کی کثیر تعداد کی موجودگی میں سپردخاک کردیے گئے۔
وہ بالکل ٹھیک تھے، انتقال کے روز نوئیڈا میں جناب رفعت سروش کے گھر ایک تقریب میں شرکت کے لیے گئے تھے، شام کو واپس آئے توکچھ بے چینی محسوس کی، رات تک طبیعت زیادہ خراب ہوئی تو اسپتال میں داخل کیے گئے جہاں روح قفس عنصری سے پرواز کرگئی، ان کی صحت بہتر رہتی تھی مگر اس سے پہلے بھی ایک بار دل کا دورہ پڑچکا تھا، یہ دوسرا دورہ جان لیوا ثابت ہوا۔
سحر صاحب مارن پیٹ ضلع محبوب نگر (آندھراپردیش) میں ۱۹؍ فروری ۱۹۳۷؁ء کو ایک متوسط گھرانے میں پیدا ہوئے تھے محبوب نگر ہائی اسکول کے اردو میڈیم اسکول سے میڑک کیا، مزید تعلیم کے لیے حیدرآباد گئے، چادرگھاٹ کالج سے انٹر کیا اور کالج میگزین کے اڈیٹر ہوئے، پھر عثمانیہ یونیورسٹی حیدرآباد کے آرٹس کالج سے بی۔اے کیا، ٹیوشن سے تعلیمی اخراجات پورا کرتے تھے، بی۔اے کرنے کے بعد حیدرآباد کے ایک قدیم اور مشہور اسکول اشرف المدارس میں ٹیچر ہوگئے مگر جلد ہی یہ ملازمت چھوڑ کر ایم۔اے کرنے کے لیے یونیورسٹی میں داخلہ لیا، مجلہ عثمانیہ کے اڈیٹر بھی ہوئے، اسی زمانے میں انہیں ماہر لسانیات پروفیسر مسعود حسین خاں سے تلمذ کا فخر حاصل ہوا، جو سحر صاحب کو ان کے اخلاص اور اردو سے دلچسپی کی بنا پر بہت عزیز رکھتے تھے، باگاریڈی صاحب سے بھی سحر صاحب ان کی اردو دوستی کی وجہ سے بہت قریب ہوگئے تھے، ایم۔اے کرنے...

Review of the Jihadi and Mystical Services of Maulana Muhammad Shoaib Tordheri

Allah has promised to protect Islam. That is why Allah created scholars and Sufis in different periods that served Islam and preached Islam. The preaching and propagation of Islam in India is due to these Sufis. They left their territories and came to India. One of these scholars and Sufis is Maulana Muhammad Shoaib Tordheri, known as Shaikh Sahib. He was the Shaikh of Moulana Abdul Ghafoor (Swat Babaji), that is the most famous Sufi in Swat Khyber Pakhtonkhwa and Shaikh of the Moulana Abdul Rahim Saharan Puri.  Moulana Muhammad Shoaib was not a scholar and sufi, he was also a mujahid. He was martyred while fighting against the Sikhs in 1823 A.D. He wrote a book about Sufism. In his book he wrote about seeratul Nabi and companion of Prophet Muhammad S.A.W. After that he discussed circumstances and sayings of Sufis of the all four series (Naqshbandia, Qadria, Cheshtia and Saharwardia) of Sufism. He also described the attributes of the perfect Peer.

Effect of Salicylic Acid and Gibberellic Acid on Morphological and Physiological Changes in Drought-Stressed Wheat Triticum Aestivum L. Crop

Global warming and uneven climatic change have augmented the drought prevalence. These dilemmas are enforcing the agri- scientist to develop some long term future policies. The aim of present study was to examine the method for improved growth and development of wheat under the premises of drought. Five wheat cultivars were used to investigate the consequence of drought on plants and it was also investigated that how hormonal priming can be helpful to cope drought. Seed of these wheat cultivars were primed in 10-4 M Gibberellic acid and Salicylic acid concentration. The response of antioxidant enzymes was variable among the non primed and primed seeds for all studied genotypes. Shahkar had the highest protease activity in primed seeds while AARI-11 had the highest amylase activity. Similarly, AARI-11, Shahkar and Chakwal-50 had the highest Superoxide dismutase activity while Shahkar and Pakistan-13 had the highest peroxidase activity. Cell membrane thermo stability, proline and relative water contents were decreased under drought stress. Hormonal priming with Gibberellic acid and Salicylic acid improved the physiological response and antioxidant enzymes activities in some genotypes under both conditions. Yield and its contributing traits yield components were lessened under the effect of drought. FSD-08 and Pakistan-13 showed maximum grain yield during control and drought condition. Priming increased the grain yield in all varieties. Grain quality characters were noticeably affected under drought stress. Hormonal seed priming was able to maintain the grain quality by minimizing the adverse effects of drought. FSD-08 was able to maintain the grain quality under normal and stress conditions. xx Drought is unavoidable under changing climatic scenario however famine can be avoided. The second experiment was conducted in order to know the mechanism of trans-generational effect of elevated carbon dioxide on winter wheat. Seeds obtained from the previous generations of ambient and elevated carbon dioxide were regrown under ambient (400ppm) and elevated (800ppm) within the green house. Drought stress was imposed for 4 days during anthesis stage and then plants were re-watered. Flag leaves were used to analyze the activities of enzymes involved in carbohydrate metabolism and antioxidant enzymes. This transgenerational effect and its enzymatic basis were not investigated previously. Results showed that glycolytic intermediates and antioxidants were enhanced under elevated carbon which ultimately increased yield. Trans-generational effect indicated that seeds had stress memory and thus maintained the effect of previous exposure. We found within source (leaf) cytoplasmic invertases, sucrose synthase, catalase and ascorbate peroxidase activities were decreased while the activity of cell wall invertases was increased under drought and elevated carbon. Similarly, the activities of glucose-6-phosphate dehydrogenase, superoxide dismutase, ascorbate peroxidase and dialyzed peroxidase were decreased under drought and elevated carbon within the sink (spike). Likewise, the activities of sucrose synthase in the source and aldolase in the sink were increased upon re-watering indicating that water is playing an important role to activate these enzymes. Similarly, lower yield was recorded under ambient carbon dioxide. These results indicate that high metabolism of sucrose synthase within the source; aldolase and glucose-6 phosphate dehydrogenase within the sink can be helpful to mitigate the drought stress under elevated carbon dioxide.