Search or add a thesis

Advanced Search (Beta)
Home > Studies on Metallo-Resistant Microorganisms for Remediation and Monitoring of Heavy Metals in Industrial Waste

Studies on Metallo-Resistant Microorganisms for Remediation and Monitoring of Heavy Metals in Industrial Waste

Thesis Info

Access Option

External Link

Author

Din, Ghufranud

Program

PhD

Institute

Quaid-I-Azam University

City

Islamabad

Province

Islamabad.

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Microbiology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12503/1/Ghufranud%20microbiology%202019%20qau%20isb%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727303220

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


In the current study, we investigated the role of metallotolerant microorganisms in remediation and detection of heavy metals like cadmium and mercury. Metallotolerant microorganisms were isolated from metal polluted soil. Bacterial isolates were screened for maximum mercury tolerance and isolate exhibiting highest mercury tolerance was selected for mercury bioreporter construction. Fungal isolates were screened for cadmium tolerance and organic acid production to select most putative strains for heavy metals bioremediation through bioleaching and biosorption. Selected bacterial and fungals isolates were identified by 16S rRNA and internal transcribed spacer (ITS) sequence phylogenetic analysis, respectively. Optimal growth conditions like pH and temperature of selected isolates were also studied. Carbon source was optimized to produce maximum amount of various organic acids that were characterized and quantified through high performance liquid chromatography (HPLC) and applied for leachig of heavy metals from metal contaminated soil. Elemental analysis in the entire study was carried out through atomic absorption spectroscopy. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) was performed to evaluate biosorption of cadmium by selected fungal strain. The localization of taken up cadmium by resistant fungi was studied through transmission electron microscopy (TEM) and electron energy loss specroscopy (EELS). The mercury resistant bacterial isolate DWH4 was engineered to serve as a bioluminescent bioreporter for mercury toxicity by linking its merR-like gene and promoter sequence to a reorganized luxABCDE gene cassette from Photorhabdus luminescens through recombinant DNA technology. A total of 10 bacterial and 13 fungal strains were isolated from soil samples collected from Misri Shah and Daroghawala. Out of 10 bacterial isolates, strain DWH4 exhibited highest tolerance to mercury and was selected for construction of mercury bioreporter cell. Among 13 fungal strains, isolate FMS1 was observed to produce maximum concentration of organic acids and was selected for bioleaching of metals. Whereas isolate FMS2, a highly cadmium resistant isolate was selected for cadmium biosorption. Sequence analysis of the 16S rRNA of bacterial isolate DWH4 was 99% similar to Enterobacter cloacae (MK500875). Sequence analysis of internal transcribed spacer regions indicated that fungal isolate FMS1 has 99% similarity to Aspergillus tubingensis (MK026408) and FMS2 was 99% similar to Penicillium chrysogenum (MH865997). The bacterial strain DWH4 could grow at wide range of temperature (20–40°C) and pH (6–9) with optimum growth at 30°C and pH 7.0. The fungal strains could grow at wide range of temperature (15–40°C) and pH (3–12) with optimum growth temperature 30°C and pH 5.0 and 6.0 for A. tubingensis FMS1 and P. chrysogenum FMS2, respectively. Three organic acids were produced with highest concentration of gluconic acid (263 mM) followed by oxalic acid (22mM) and fumaric acid (0.34mM) at day 12 and sucrose was considered as the ideal carbon source. The highest leaching efficiency with organic acids from A. tubingensis strain FMS1 was observed for cadmium (58%), cobalt (53%) and nickel (52%) within 28 days. Cadmium removal capacity of P. chrysogenum strain FMS2 was observed 49% in 15 days. Distinct white patches were seen in SEM with clear peaks of cadmium in EDX analysis of white patches indicating biosorption of cadmium by P. chrysogenum strain FMS2. The TEM and EELS of P. chrysogenum strain FMS2 revealed cytoplasmic localization of cadmium. The constructed bioluminescent bioreporter for mercury detection, designated as E. cloacae strain DWH4lux, detected mercury (HgCl2) at a minimum concentration of 0.2 µg/L with a linear response profile being maintained between a range of 0.4–1600 µg/L (R2 = 0.9604) with a peak bioluminescent response occurring within one hour after exposure. No significant synergistic or antagonistic influences were observed on the bioluminescent response by other contaminating metal elements. E. cloacae strain DWH4lux was also demonstrated to detect mercury effectively in artificially contaminated water sample with linear correlation (R2=0.9623). This study provides new insight into the recruitment of metallotolerant bacteria from environmental samples for mercury bioreporter development and their potential for in-field biosensing applications. The biosorption and bioleaching ability of the newly isolated fungal strains can also be helpful in redemption of polluted soil especially with trace toxic metals such as in case of compost and sewage sludge and bioremediation of industrial effluents containing cadmium to minimize its release to environment.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

90. Al-Balad/The City

90. Al-Balad/The City

I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

90:01
a. I swear by this City,

90:02
a. and you – O The Prophet - are a resident of this City,

90:03
a. as well as any parent and anyone born of him.

90:04
a. Indeed, WE created human being in stages of difficulty and hardship.

90:05
a. Now that he is in the midst of blessings,
b. does he think that no one will have power over him to hold him accountable?

90:06
a. For he boasts:
b. ‘I have squandered a great deal of wealth!’

90:07
a. Does he think that no one ever watches him with regard to what he has expended?
b. Indeed, Allah watches him all the time!

90:08
a. Have WE not given two eyes to him,
b. to see and recognize the truth?

90:09
a. And a tongue, and a pair of lips,
b. to speak and advocate the truth?

90:10
a. And have WE not showed him the two ways:
b. virtue and vice; good and evil?

Surah 90 * Al-Balad 737

90:11
a. Yet he makes no attempt to the steep/uphill way, i.e., good, virtue.

90:12
a. And what will enable you to comprehend the steep/uphill way?

90:13
a. The setting free of the neck from the burden of bondage or debt,

90:14
a. or, giving food at the time of one’s hunger/impoverishment,

90:15

THE PHILOSOPHY OF CONTEMPORARY EDUCATION AND ITS IMPLICATIONS FOR THE DEVELOPMENT OF ISLAMIC EDUCATION

The title of this research is Theoretical Debate over the Philosophical Roots of Contemporary Education and Its Implications for the Development of Islamic Education. The purpose is to disentangle, to explore and to discuss the foundations of theoretical roots of educational philosophy and its relational consequences with Islamic education. The issues raised are: 1). How is the philosophical foundation of education and its relationship to philosophy and education? 2). What are the Philosophical Roots of Educational Theory and Its Implications for Curriculum, Teaching and Learning? 3). What are the implications for the development of Islamic education? The type of research is Library Research which uses philosophical and pedagogical approach, with descriptive method. The findings of this study are: a) the study of the key philosophical ideas that influence thought and development of education are aspects of metaphysics, epistemology and axiology. Without these three philosophical foundations, science and its various disciplines will fail; b) the relationship between philosophy and education are like two sides of one coin that cannot be separated, both give and take in the context of mutualism. So the existence of philosophy in education is a necessity; c) The theoretical debate over the roots of the philosophy of education requires the development of Islamic education.

Majoriztion and its Applications

The notion of majorization arose as a measure of the diversity of the components of an n-dimensional vector (an n-tuple) and is closely related to convexity. Many of the key ideas relating to majorization were discussed in the volume entitled Inequalities by Hardy, Littlewood and Polya (1934). Only a relatively small number of researchers were inspired by it to work on questions relating to majorization. After the volume entitled Theory of Majorization and its Applications (Marshall and Olkin, 1979), they heroically had shifted the literature and endeavored to rearrange ideas in order, often provided references to multiple proofs and multiple viewpoints on key results, with reference to a variety of applied fields. For certain kinds of inequalities, the notion of majorization leads to such a theory that is sometime extremely useful and powerful for deriving inequalities. Moreover, the derivation of an inequality by methods of majorization is often very helpful both for providing a deeper understanding and for suggesting natural generalizations. Majorization theory is a key tool that allows us to transform complicated non-convex constrained optimization problems that involve matrix-valued variables into simple problems with scalar variables that can be easily solved. In this PhD thesis, we restrict our attention to results in majorization that directly involve convex functions. The theory of convex functions is a part of the general subject of convexity, since a convex function is one whose epigraph is a convex set. Nonetheless it is an important theory, which touches almost all branches of mathe- matics. In calculus, the mean value theorem states, roughly, that given a section of a smooth curve, there is a point on that section at which the derivative (slope) of the viiviii curve is equal (parallel) to the ”average” derivative of the section. It is used to prove theorems that make global conclusions about a function on an interval starting from local hypotheses about derivatives at points of the interval. In the first chapter some basic results about convex functions, some other classes of convex functions and majorization theory are given. In the second chapter we prove positive semi-definite matrices which imply exponen- tial convexity and log-convexity for differences of majorization type results in discrete case as well as integral case. We also obtain Lypunov’s and Dresher’s type inequalities for these differences. In this chapter both sequences and functions are monotonic and positive. We give some mean value theorems and related Cauchy means. We also show that these means are monotonic. In the third chapter we prove positive semi-definite matrices which imply a surprising property of exponential convexity and log-convexity for differences of additive and multiplicative majorization type results in discrete case. We also obtain Lypunov’s and Dresher’s type inequalities for these differences. In this chapter we use mono- tonic non-negative as well as real sequences in our results. We give some applications of majorization. Related Cauchy means are defined and prove that these means are monotonic. In the fourth chapter we obtain an extension of majorization type results and ex- tensions of weighted Favard’s and Berwald’s inequality when only one of function is monotonic. We prove positive semi-definiteness of matrices generated by differ- ences deduced from majorization type results and differences deduced from weighted Favard’s and Berwald’s inequality. This implies a surprising property of exponen- tial convexity and log-convexity of these differences which allows us to deduce Lya- punov’s and Dresher’s type inequalities for these differences, which are improvements of majorization type results and weighted Favard’s and Berwald’s inequalities. Anal- ogous Cauchy’s type means, as equivalent forms of exponentially convexity and log- convexity, are also studied and the monotonicity properties are proved. In the fifth chapter we obtain all results in discrete case from chapter four. Weix give majorization type results in the case when only one sequence is monotonic. We also give generalization of Favard’s inequality, generalization of Berwald’s inequal- ity and related results. We prove positive semi-definiteness of matrices generated by differences deduced from majorization type results and differences deduced from weighted Favard’s and Berwald’s inequality which implies exponential convexity and log-convexity of these differences which allow us to deduce Lyapunov’s and Dresher’s type inequalities for these differences. We introduce new Cauchy’s means as equiva- lent form of exponential convexity and log-convexity. In the sixth chapter we prove positive semi-definiteness of matrices generated by dif- ferences deduced from Popoviciu’s inequalities which implies a surprising property of exponential convexity and log-convexity of these differences which allows us to deduce Gram’s, Lyapunov’s and Dresher’s type inequalities for these differences. We intro- duce some mean value theorems. Also we give the Cauchy means of the Popoviciu type and we show that these means are monotonic.