عصرحاضر کے مسائل کے بارے فتاوی میں احتیاط کے منہج کی عصری معنویت و اہمیت اسباب اور اصول و ضوابط

Fatwa is the easiest tool to explore the religious status of any newer problem faced by the public, though it is non-binding theory  by its nature still people rely on that and get it from any renowned scholar to for the sake of awareness and to support their religious background. The ultimate result in fatwa depends on the methodology adopted in it. Alike methodologies of  tashdeed and tasahul the precautionary methodology has also been practiced in the earlier stages of Islam  when the issues were not much complicated but in the era  we live in the newer are much complicated which need this methodology to be practiced. This study throws light on the concept of such methodology, on its reasons and principles. It concludes that the precautionary methodology of fatwa is based on shariah rules and its is admissible by its nature but when this methodology becomes the way to save a compulsory thing then it becomes compulsory and when adoption of this methodology becomes to strengthen a Haram action then adoption of this methodology becomes Haram.

Anti-Cancerous Metabolites and Extracellular Enzyme Production by Endophytic Penicillium and Paecilomyces Strains

Fungal endophytes colonize an important niche within the plants through secretion of secondary metabolites. The metabolites and extracellular enzymes produced by endophytic fungi regulate the growth of the host plant and contribute in defence mechanisms.The medicinal plants Caralluma acutangula and Boswellia sacra were used for the isolation of endophytic fungi. The endophytic fungi were identified as Penicillium citrinum, Paecilomyces variotii, Aspergillus nidulans, Fusarium oxysporum, Epicucum nigram, Penicillium purpurogenum, Penicillium spinulosum, Aspergillus caespitosus, Phoma and Alternaria sp. and were assessed for their potential to produce anti-cancerous metabolites by performing MTT assay and extracellular enzymes such as cellulases, phosphatases and glucosidases in growth media. P. variotii, P. citrinum and F. oxysporum showed significantly higher amount of phosphatases and glucosidases as compared to other strains. Additionally, P. variotii and F. oxysporum showed significantly higher potential of indole acetic acid production (tryptophan-dependent and independent pathways). ACC (1-Aminocyclopropane-1-carboxylate) deaminase results showed that P.citrinum, P. purpurogenum and P. Variotii had shown prominent ACC deaminase activity (300 nmol α- ketobutyrate mg-1h-1). Fluorescence-based MUB (4-methyl umbelliferone) standards were used to analyze the presence of extracellular enzymes glucosidase, phosphatase and cellulase. The bioactive secondary metabolites from endophytic P. citrinum also revealed some prominent results by performing MTT assay on breast cancer cell line (MCF-7). The current study concludes that these fungi are producing bioactive constituents that could provide unique niche of ecological adaptation by symbiosis and greatly contributing to the healthy life of their host plant. However, some of the endophytic fungi offer a great potential to produce anti-cancerous metabolites and extracellular enzymes.