Search or add a thesis

Advanced Search (Beta)
Home > Transformation and Expression Studies of Multiple Frost Tolerant Genes in Solanum Tuberosum L.

Transformation and Expression Studies of Multiple Frost Tolerant Genes in Solanum Tuberosum L.

Thesis Info

Access Option

External Link

Author

Khadija Aaliya

Program

PhD

Institute

University of the Punjab

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Molecular Biology

Language

English

Link

http://prr.hec.gov.pk/jspui/bitstream/123456789/12389/1/khadija%20aaliya%20molecular%20biology%202019%20uop%20lhr%20prr.pdf

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676727628053

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Potato crop is vulnerable to a number of biotic and abiotic stresses. Among abiotic stresses, frost may cause devastating effects on quantity as well as quality of yield. In Pakistan, 75% of potato is cultivated as autumn season crop sown in Sep-Oct and harvested in Jan-Feb. This crop undergoes frost during tuber formation stage resulting in significant loss of production. The aim of the present research was the expression studies of Ice Recrystallization Inhibition Protein (IRI3) from Lolium perenne, Antifreeze protein (AFP) from Daucus carota and Zinc Finger protein (ZnFP) from Gossypium hirsutum under CaMV35S Promoter in potato variety Diamant to enhance the tolerance against cold stress. Expression of these genes in potato was enhanced under the influence of 58 base pair 5’-untranslated region of Alcohol Dehydrogenase Gene (ADH1) as a Translational enhancer. To study sub-cellular Localization of these Proteins, gene cassettes were fused with GFP gene and transformed in model plant Arabidopsis thaliana. Confocal Microscopy revealed the expression of these proteins in the cytoplasm of the cells of Germinating Arabidospsis seedling under stress conditions. To achieve the objective of this study, genes were cloned in plant expression vector pCAMBIA 1301 under the control of 35S constitutive promoter and then transformed into potato plants through Agrobacterium mediated transformation system. Transgenic plants were confirmed with GUS histochemical analysis, PCR and southern Blot analysis. Quantitative expression in Positive transgenic plants was increased under stress conditions. As high as 20.41 fold increase of IRI3 gene expression was observed under stress conditions. The expression of ZnFP and AFP was also increased 16.1 folds and 10.2 folds respectively under stress conditions. Positive transgenic plants exhibited reduction in ion leakage percentage. Ion leakage percentage was observed as 59.41% for IRI3, 65.34% for ZnFP and 70.21% for AFP as compared to 83.21% of control. Plant survival rate was enhanced significantly in transgenic plants. It can be concluded from the results that these genes induced tolerance against low temperature. Thus, use of DNA recombinant technology to combat abiotic stress could be promising for increasing the production of the potato crop in Pakistan.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

ڈاکٹرراجندر پرشاد

ڈاکٹر راجندر پرشاد
خدا کاشکر ہے جو سفر ۹؍ ستمبر ۶۲ء کوشروع ہواتھا وہ بخیر وخوبی ۲۳؍ مئی ۶۳ء کوپورا ہوگیا۔ اس مدت میں علم وادب اورملک وقوم کی بعض بڑی نامور اورمحبوب شخصیتیں ہم سے ہمیشہ کے لیے جدا ہوگئیں،ان میں سابق صدر جمہوریہ ڈاکٹر راجندر پرشاد،ڈاکٹر محی الدین زورؔ،خان بہادر مولوی محمدشفیع سابق پروفیسر عربی پنجاب یونیورسٹی لاہور، مولانا سعید انصاری اور جناب شفیق جونپوری خاص طور پر لائقِ ذکرہیں۔
گذشتہ چندماہ سے مکاتیب کناڈانے نظرات کے صفحات پرقبضہ کررکھا تھا اس لیے ان حضرات کی رسمِ تعزیت برہان میں حسب معمول ادانہ ہوسکی، جس کاافسوس ہے۔اوّل الذکر ملک کی تحریکِ آزادی کے بلند پایہ لیڈر، پہلے صدر جمہوریہ ہندہونے کے ساتھ ساتھ اخلاق واوصافِ ذاتی کے اعتبار سے بھی غیر معمولی شخصیت اورکردار کے انسان تھے۔ سادگی، مذہبیت، خلوص، رواداری اور مروت و وضعداری اُن کے خاص اوصافِ کمال تھے اوراِس حیثیت سے وہ صحیح معنی میں گاندھی جی کے نقشِ قدم پر چلنے والے تھے، علاوہ ازیں انگریزی زبان کے نامور مصنف اور اُردو ،فارسی کے فاضل بھی تھے۔ [جون ۱۹۶۳]

العلامة شائسته جل و خدماته العلمية الدينية السياسية

This article highlights the religious, literary and political work of Moulana ShÉista Gul famous by the name of Mathe Mulla of Khyber Puktun Khawa (1303 A. H-1401A. H. 1886A. D-1981A. D MardÉn) He was well-known scholar of Qura’n, ×adith and Fiqah, his great contribution was to promote political awareness in the community with the concept ofTwo Nations Theory by his affiliation with Muslim League under the leadership of Quaid-eAÐam Muhammad Ali JinnaÍ. He was the convener of Jamiat ul AÎfia which was organized by the eminent religious leaders of that time to implement Sharia law in Pakistan. He was devoted to make Islam a living reality during his life time, his literary work is aimed to improve human communication and develop a better understanding among the people of this region. His monumental work was originally in Pashto widely spoken and understood in the North West of Pakistan. He was teaching and preaching Islam nearly in a span of seventy years. The main objective was to alleviate the deteriorating conditions of the Muslim society and to defend it from external threats. He emphasized to bring change and correct the wrong believes and detrimental actions. His theological doctrines influenced his followers and decedents who promoted his mission in the light of writings and ideas expressed by him.

Productivity of Maize Zea Mays L. under Various Agro-Management Techniques

Studies on productivity of maize hybrid under varying row spacing, time and method of nitrogen application and irrigation methods to maize were conducted at Agronomic Research Farm, University of Agriculture, Faisalabad, Pakistan during autumn 2006 and 2007. Two experiments were conducted. In Experiment I, row spacing; S 1 (60 cm spaced single rows), S 2 (90 cm spaced double row strips), S 3 (90 cm spaced single rows) and S 4 (135 cm spaced double row strips) were randomized in main plots while two methods of nitrogen application; N 1 (broadcast) and N 2 (band placement) were placed in sub plots. In Experiment II, three methods of irrigation application; I 1 (flood irrigation, I 2 (each furrow irrigation) and I 3 (alternate furrow irrigation) were kept in main plots while time of nitrogen application; N 1 (control), N 2 (1⁄2 N at sowing and 1⁄2 at Ist irrigation), N 3 (1⁄2 N at sowing and 1⁄2 at 45 DAS) and N 4 ( 1 / 3 N at sowing + 1 / 3 at Ist irrigation and 1 / 3 at 45 DAS) were randomized in sub plots. Results revealed that maize crop planted in 2006 performed better than that grown in 2007. On an average of two years, the crop planted at 90 cm spaced double row strips produced higher grain yield (6.31 t ha -1 ) which was 17.91, 10.35 and 8.24% higher compared to 60 cm spaced single rows, 90 cm spaced single rows and 135 cm spaced double row strips along with more number of grains cob -1 , higher 1000-grain weight and more grain weight per cob. Days taken to 50 % tasseling and silking were delayed in S 1 over rest of three row spacing. S 2 exhibited higher LAI, TDM, CGR and NAR followed by S 4 . Crude starch and protein content in grains were also found maximum in S 2 . Whereas the band placement of nitrogen produced higher grain yield (two years average 6.04 t ha -1 ), more number of grains per cob, number of cobs per plant, higher 1000- grain weight and more grain weight per cob over broadcast method. Band placement of N also produced taller plants and delayed tasseling and silking over broadcast. Similarly, higher LAI, TDM, CGR and NAR were recorded in band placement of nitrogen than broadcast. Moreover, the band placement of N was also found to be superior with maximum crude starch and protein content in grains over broadcast. On an average of two years, the highest net income (Rs. 72376 ha -1 and Rs. 67226 ha -1 ) was recorded in 90 cm spaced xviiidouble row strips (I 2 ) and band placement of nitrogen application (N 1 ), respectively. In 2 nd experiment, on an average of two years the irrigation to each furrow (I 2 ) produced more grain yield (6.08 t ha -1 ), harvest index, number of cobs plant -1 , grain weight cob -1 and higher 1000- grain weight compared to flood irrigation (I 2 ) and alternate furrow irrigation (I 3 ) . Similarly, the crop taken more days to tessel and silking in I 2 . I 2 exhibited higher LAI, TDM, CGR and NAR than rest of two irrigation methods. On an average of two years, three equal splits of (N 3 ) viz, 1/3 N at sowing + 1/3 at Ist irrigation and 1/3 at 45 DAS produced higher grain yield (5.72 t ha -1 ), more harvest index, more number of grains cob -1 , more number of cobs plant -1 , higher 1000- grain weight, more grain weight cob -1 . Days taken to tasseling and silking were delayed in N 3 . Likewise, higher LAI, TDM, CGR and NAR were recorded in case of N 3 . Crude starch and protein content in grains were found to be more in N 3 followed by N 2 and N 1 . Maximum nitrogen use efficiency (two years average 7.56 kg kg -1 and 8.93 kg kg -1 ) was recorded in irrigation to each furrow and N 3 , respectively. On average of two years, the I 2 x N 3 treatment combination was found superior with higher grain yield (6.75 t ha -1 ), nitrogen use efficiency (10.94 kg kg -1 ) and water use efficiency (16.63 kg ha -1 mm - 1 ) amongst many others. The irrigation to maize crop by each furrow with nitrogen application in three equal splits (at sowing, Ist irrigation and at 45 DAS) gave maximum net income amounting to Rs. 80509 ha -1 . For getting higher yield and economic returns, maize hybrids should be preferably planted at 90 cm spaced double row strips with three equal splits along with band application of nitrogen in three equal splits.