101. Al-Qari’ah/The Calamity
I/We begin by the Blessed Name of Allah
The Immensely Merciful to all, The Infinitely Compassionate to everyone.
101:01
a. The Calamity!
101:02
a. What will be The Calamity?
101:03
a. And what may enable you to comprehend The Calamity?
101:04
a. The Calamity is going to happen at the dreadful Time when human beings will seem like moths scattered all around in confusion,
101:05
a. and the mountains will be like tufts of wool.
101:06
a. As for the one whose scales of true faith and deeds and dealings of righteousness will be heavier in weight,
101:07
a. he will be in a life, pleasing - full of joy and contentment.
101:08
a. But as for the one whose scales of true faith and deeds and dealings of righteousness will be lighter in weight,
101:09
a. his mother will be the womb of the bottomless Pit.
101:10
a. And what may enable you to comprehend what that Pit is?
101:11
a. It will be a Fire blazing fiercely!
The present study aims at exploring positive psychological capital in the verses of Qur’an. Positive psychology is the latest advancement in the field of psychology which focuses on improving the well-being of society. Positive psychological capital refers to the combination of overall qualities of positive psychology that contributes to the well-being and mental health. The present study is based on the content analysis of the verses of Qur’an. Content analysis comprises of three steps including identifying the categories or themes, dividing the information into units or parts and finally rating all the themes in all units. In the first step the researchers identified 41 themes from Qur’an by using committee approach and reading the verses between the lines. All these categories were identified by keeping in view the underlying themes of positive psychology. In the second step 30 units were devised from Qur’an by considering each Part as a single unit. The categories included behavior modification, belief in divine help, brotherhood, bravery, contentment, civility, credibility, encouragement of virtue, emotional regulation, excellence, forgiveness, generosity, gratitude, honesty, hopefulness, humility, justice, knowledge, lawful spending, learning, meaningfulness, mindfulness, moderation, obedience, patience, peace, determination, positivity, prosperity, repentance, resilience, reward, self-actualization, self-awareness, self-control, sincerity, social leadership, truthfulness, trust, and wisdom. Results suggest that the most prominent category in Qur’an is the reward. Validity of the study was maintained through the selection of the themes with the help of committee approach. Reliability of the scoring system was maintained through partial inter-rater reliability. Overall the present research has many implications in the positive psychology of religion.
Successful repair of DNA double strand breaks is crucial; failure of which can lead to tumourigenesis including breast/ovarian cancer. BRCA2 (breast cancer susceptibility protein type 2) and RAD51 from different specie interact in a process called homologous recombination (HR) during the repair of such kind of insults to DNA. BRCA2 being a large protein molecule has different sites of interaction for other proteins like DMC1 (disruption of meiotic control 1). In the present study, we have purified a distinct region in human BRCA2 close to DMC1 binding site which has been shown to undergo CDK (cyclin dependent kinase) based phosphorylation in our kinase assays. Characterization involving dynamic light scattering, limited proteolysis and circular dichroism spectroscopy techniques revealed this region to be mostly disordered. We have also attempted the successful purification of DMC1 (different from the previous method of its purification) employing C-terminus of DMC1 as binding partner for increased solubility. Further studies included the characterization of Trypanosoma brucei BRC repeats (TbBRC) and Rad51 interaction. We observed no interaction of Trypanosoma brucei Rad51 (TbRad51) with its BRC repeats upto 7 BRC repeats based on our in vitro pull down assays. The comparison of human RAD51 and TbRad51 structures highlighted critical differences of residues at hydrophobic cavities for the binding of phenylalanine from BRC repeat. We predicted that TbBRC repeats, collectively, might have WD-40 β-propeller structure. The purification of Trypanosoma brucei 7 BRC repeats rendered this protein highly soluble and stable based on circular dichroism spectroscopy which also revealed the protein having poly-proline structure, a characteristic of unordered proteins. Moreover, we have predicted multiple phosphorylation sites in TbRad51 and compared them with other eukaryotic Rad51 orthologues to get insights into a possible regulatory mechanism for Rad51.